Hwang Jung Ho, Lee Sang Young, Choi Jungil
Department of Electrical and Computer Engineering, Undergraduate School, Michigan State University, 426 Auditorium Road, East Lansing, MI 48824, USA.
Department of Mechanical Engineering, Undergraduate School, Kookmin University, 77 Jeongneung-ro, Seongbuk-gu, Seoul 02707, Korea.
Antibiotics (Basel). 2021 Mar 13;10(3):300. doi: 10.3390/antibiotics10030300.
Antimicrobial resistance has become a major problem in public health and clinical environments. Against this background, antibiotic susceptibility testing (AST) has become necessary to cure diseases in an appropriate and timely manner as it indicates the necessary concentration of antibiotics. Recently, microfluidic based rapid AST methods using microscopic analysis have been shown to reduce the time needed for the determination of the proper antibiotics. However, owing to the inoculum effect, the accurate measurement of the minimal inhibitory concentration (MIC) is difficult. We tested four standard bacteria: , against five different antibiotics: piperacillin, cefotaxime, amikacin, levofloxacin, and ampicillin. The results showed that overall, the microfluidic system has a similar inoculum effect compared to the conventional AST method. However, due to the different testing conditions and determination protocols of the growth of the microfluidic based rapid AST, a few results are not identical to the conventional methods using optical density. This result suggests that microfluidic based rapid AST methods require further research on the inoculum effect for practical use in hospitals and can then be used for effective antibiotic prescriptions.
抗菌药物耐药性已成为公共卫生和临床环境中的一个主要问题。在此背景下，抗生素敏感性测试（AST）对于及时恰当地治愈疾病变得至关重要，因为它能表明所需的抗生素浓度。最近，基于微流控技术并使用显微镜分析的快速AST方法已被证明可缩短确定合适抗生素所需的时间。然而，由于接种物效应，准确测量最低抑菌浓度（MIC）较为困难。我们针对四种标准细菌，测试了五种不同的抗生素：哌拉西林、头孢噻肟、阿米卡星、左氧氟沙星和氨苄西林。结果表明，总体而言，与传统AST方法相比，微流控系统具有相似的接种物效应。然而，由于基于微流控的快速AST生长的测试条件和测定方案不同，一些结果与使用光密度的传统方法并不相同。这一结果表明，基于微流控的快速AST方法需要针对接种物效应进行进一步研究，以便在医院实际应用，进而可用于有效的抗生素处方。
