Kimron Veterinary Institute, Bet Dagan 50250, Israel.
The Israeli Wildlife Hospital and Safari, Zoological Center, Tel Aviv 5225300, Israel.
Viruses. 2021 Mar 4;13(3):407. doi: 10.3390/v13030407.
During 2019, five carcasses of juvenile Egyptian fruit bats () were submitted to the Kimron Veterinary Institute. These bats exhibited typical poxvirus like lesion plaques of different sizes on the skin, abdomen and the ventral side of the wings. Clinical and histopathological findings suggested a poxvirus infection. Infectious virus was isolated from skin swabs, skin tissue and tongue of the dead bats and was further confirmed to be a Poxvirus by molecular diagnosis using PCR with pan-chordopoxviruses primers. All the dead bats were found positive for two Poxvirus genes encoding a metalloproteinase and DNA dependent DNA polymerase. In this study, a novel real time quantitative PCR (qPCR) assay was established to further confirmed the presence of specific poxvirus viral DNA in all pathologically tested tissues. Moreover, according to sequence analysis, the virus was found to be highly similar to the recently discovered Israeli Pox Virus (IsrRAPXV).
2019 年期间，五具幼年埃及果蝠（）的尸体被送到 Kimron 兽医研究所。这些蝙蝠的皮肤、腹部和翅膀腹侧呈现出典型的痘病毒样大小不一的病变斑块。临床和组织病理学检查结果提示痘病毒感染。从死蝙蝠的皮肤拭子、皮肤组织和舌部分离出传染性病毒，并通过使用泛痘病毒引物的 PCR 进行分子诊断进一步证实为痘病毒。所有死亡的蝙蝠均在编码金属蛋白酶和 DNA 依赖性 DNA 聚合酶的两个痘病毒基因上呈阳性。在这项研究中，建立了一种新型实时定量 PCR（qPCR）检测方法，以进一步证实所有经过病理检测的组织中存在特定的痘病毒病毒 DNA。此外，根据序列分析，该病毒与最近发现的以色列痘病毒（IsrRAPXV）高度相似。
