Zavan Barbara, Gardin Chiara, Guarino Vincenzo, Rocca Tiberio, Cruz Maya Iriczalli, Zanotti Federica, Ferroni Letizia, Brunello Giulia, Chachques Juan-Carlos, Ambrosio Luigi, Gasbarro Vincenzo
GVM Care & Research, Maria Cecilia Hospital, 48033 Cotignola, Italy.
Translational Medicine Department, University of Ferrara, 44123 Ferrara, Italy.
Nanomaterials (Basel). 2021 Mar 16;11(3):751. doi: 10.3390/nano11030751.
Electrospun fibers have attracted a lot of attention from researchers due to their several characteristics, such as a very thin diameter, three-dimensional topography, large surface area, flexible surface, good mechanical characteristics, suitable for widespread applications. Indeed, electro-spinning offers many benefits, such as great surface-to-volume ratio, adjustable porosity, and the ability of imitating the tissue extra-cellular matrix.
we processed Poly ε-caprolactone (PCL) via electrospinning for the production of bilayered tubular scaffolds for vascular tissue engineering application. Endothelial cells and fibroblasts were seeded into the two side of the scaffolds: endothelial cells onto the inner side composed of PCL/Gelatin fibers able to mimic the inner surface of the vessels, and fibroblasts onto the outer side only exposing PCL fibers. Extracellular matrix production and organization has been performed by means of classical immunofluorescence against collagen type I fibers, Scanning Electron-Microscopy (SEM) has been performed in order to evaluated ultrastructural morphology, gene expression by means gene expression has been performed to evaluate the phenotype of endothelial cells and fibroblasts.
results confirmed that both cells population are able to conserve their phenotype colonizing the surface supporting the hypothesis that PCL scaffolds based on electrospun fibers should be a good candidate for vascular surgery.
电纺纤维因其多种特性吸引了研究人员的广泛关注，如直径极细、三维拓扑结构、大表面积、表面灵活、良好的机械特性，适用于广泛应用。事实上，静电纺丝具有许多优点，如高比表面积、可调节的孔隙率以及模仿组织细胞外基质的能力。
我们通过静电纺丝加工聚ε-己内酯（PCL），以生产用于血管组织工程应用的双层管状支架。将内皮细胞和成纤维细胞接种到支架的两侧：内皮细胞接种到由能够模仿血管内表面的PCL/明胶纤维组成的内侧，而成纤维细胞仅接种到仅暴露PCL纤维的外侧。通过针对I型胶原纤维的经典免疫荧光法进行细胞外基质的产生和组织分析，通过扫描电子显微镜（SEM）评估超微结构形态，通过基因表达分析评估内皮细胞和成纤维细胞的表型。
结果证实，两种细胞群体都能够在定植于表面时保持其表型，支持了基于电纺纤维的PCL支架应是血管手术良好候选材料的假设。
