用于测定微流控通道中单个红细胞数量和细胞血红蛋白浓度的光学研究

Optical Investigation of Individual Red Blood Cells for Determining Cell Count and Cellular Hemoglobin Concentration in a Microfluidic Channel.

作者信息

Reichenwallner Ann-Kathrin, Vurmaz Esma, Battis Kristina, Handl Laura, Üstün Helin, Mach Tivadar, Hörnig Gabriele, Lipfert Jan, Richter Lukas

机构信息

Technologies for Precision Medicine, Siemens Healthcare GmbH, Günther-Scharowsky-Str. 1, 91058 Erlangen, Germany.

Department of Physics and Center for Nanoscience, LMU Munich, Amalienstr. 54, 80799 Munich, Germany.

出版信息

Micromachines (Basel). 2021 Mar 26;12(4):358. doi: 10.3390/mi12040358.

DOI:10.3390/mi12040358

PMID:33810262

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8066749/

Abstract

We demonstrate a blood analysis routine by observing red blood cells through light and digital holographic microscopy in a microfluidic channel. With this setup a determination of red blood cell (RBC) concentration, the mean corpuscular volume (MCV), and corpuscular hemoglobin concentration mean (CHCM) is feasible. Cell count variations in between measurements differed by 2.47% with a deviation of -0.26×106 μL to the reference value obtained from the Siemens ADVIA 2120i. Measured MCV values varied by 2.25% and CHCM values by 3.78% compared to the reference ADVIA measurement. Our results suggest that the combination of optical analysis with microfluidics handling provides a promising new approach to red blood cell counts.

摘要

我们通过在微流控通道中利用光学显微镜和数字全息显微镜观察红细胞来展示一种血液分析程序。通过这种设置，可以测定红细胞（RBC）浓度、平均红细胞体积（MCV）和平均血红蛋白浓度（CHCM）。测量之间的细胞计数变化相差2.47%，与从西门子ADVIA 2120i获得的参考值偏差为-0.26×106 μL。与ADVIA参考测量相比，测得的MCV值变化2.25%，CHCM值变化3.78%。我们的结果表明，光学分析与微流控处理相结合为红细胞计数提供了一种有前景的新方法。