Division of Vertebrate Zoology, American Museum of Natural History, New York, NY 10024, USA.
Zoology Division, National Museum of Natural History, Manila 1000, Philippines.
Viruses. 2021 Mar 26;13(4):563. doi: 10.3390/v13040563.
Diverse paramyxoviruses have coevolved with their bat hosts, including fruit bats such as flying foxes (Chiroptera: Pteropodidae). Several of these viruses are zoonotic, but the diversity and distribution of are poorly understood. We screened pooled feces samples from three colonies and assayed tissues, rectal swabs, and oral swabs from 95 individuals of 23 pteropodid species sampled at 17 sites across the Indonesian archipelago with a conventional paramyxovirus PCR; all tested negative. Samples from 43 individuals were screened with next generation sequencing (NGS), and a single collected near Flores had Tioman virus sequencing reads. Tioman virus is a bat-borne virus in the genus with prior evidence of spillover to humans. This work expands the known range of Tioman virus, and it is likely that this isolated colony likely has sustained intergenerational transmission over a long period.
多种副黏病毒与其蝙蝠宿主共同进化，包括果蝠（翼手目：蝙蝠科）。其中一些病毒是人畜共患病，但它们的多样性和分布情况知之甚少。我们对三个蝙蝠种群的粪便样本进行了混合筛选，并使用传统副黏病毒 PCR 对在印度尼西亚群岛 17 个地点采集的 23 种蝙蝠科 95 只个体的组织、直肠拭子和口腔拭子进行了检测；所有样本均为阴性。对 43 个样本进行了下一代测序（NGS）筛选，在弗洛雷斯附近采集的一个样本中检测到了蒂奥曼病毒的测序结果。蒂奥曼病毒是一种携带在属中的蝙蝠传播病毒，此前有证据表明它已经溢出到人类。这项工作扩展了蒂奥曼病毒的已知范围，并且这个孤立的种群很可能在很长一段时间内持续存在代际传播。
