Chen Jiawen, Qiu Liping, Li Qinglin, Ai Jie, Liu Haiqing, Chen Qinhui
College of Chemistry and Materials Science, Fujian Normal University, Fuzhou 350007, People's Republic of China.
Fujian Provincial Jinshan Hospital, Fuzhou 350007, People's Republic of China.
Mater Sci Eng C Mater Biol Appl. 2021 Apr;123:111958. doi: 10.1016/j.msec.2021.111958. Epub 2021 Feb 12.
It is important to control bleeding and prevent bacterial infection for the wound people. The effective way is to fabricate an asymmetric Janus matrial for realizing rapid hemostasis and promoting wound healing. Herein, mesoporous silica nanoparticles (MSN) modified by tannic acid (TA), silver nanoparticles, and calcium ions (Ca-TA-MSN@Ag) with Janus structure were prepared via redox and coordination reactions. These anisotropic snowman-like particles possess obvious chemical compartition, in which silver nanoparticles are embedding in large MSN body. During blood coagulation, TA with catechol structure acts as a vasoconstrictor. Then, Ca-TA-MSN@Ag with high specific surface area (510.62 m·g) and large pore volume (0.48 m·g) induces red blood cell aggregation to form three-dimensional network structure with fibrin. Additionally, calcium ions as clotting factor IV and negative charge of Ca-TA-MSN@Ag accelerate coagulation cascade reaction. These three synergistic effects on animal model showed that hemostatic time of Ca-TA-MSN@Ag was shortened by nearly 50% compared to that of MSN. Moreover, Ca-TA-MSN@Ag possessed good blood compatibility, biocompatibility and antibacterial activity (~99%) against E. coli and S. aureus. The anisotropic Janus particles of Ca-TA-MSN@Ag with hemostatic performance and antibacterial activity will be a promising biomaterial for designing wound dressings in clinical application.
对于伤口患者来说，控制出血和预防细菌感染很重要。有效的方法是制备一种不对称的Janus材料，以实现快速止血和促进伤口愈合。在此，通过氧化还原和配位反应制备了具有Janus结构的单宁酸（TA）、银纳米颗粒和钙离子修饰的介孔二氧化硅纳米颗粒（Ca-TA-MSN@Ag）。这些各向异性的雪人状颗粒具有明显的化学分区，其中银纳米颗粒嵌入在大的MSN主体中。在血液凝固过程中，具有邻苯二酚结构的TA作为血管收缩剂。然后，具有高比表面积（510.62 m²·g⁻¹）和大孔体积（0.48 cm³·g⁻¹）的Ca-TA-MSN@Ag诱导红细胞聚集，与纤维蛋白形成三维网络结构。此外，钙离子作为凝血因子IV以及Ca-TA-MSN@Ag的负电荷加速凝血级联反应。这三种协同作用在动物模型上显示，与MSN相比，Ca-TA-MSN@Ag的止血时间缩短了近50%。此外，Ca-TA-MSN@Ag具有良好的血液相容性、生物相容性以及对大肠杆菌和金黄色葡萄球菌的抗菌活性（约99%）。具有止血性能和抗菌活性的各向异性Janus颗粒Ca-TA-MSN@Ag将是临床应用中设计伤口敷料的一种有前景的生物材料。
