Liu Ailin, Xiao Zhixia, Wang Zhili, Lam Hon-Ming, Chye Mee-Len
School of Biological Sciences, The University of Hong Kong, Pokfulam, China.
Centre for Soybean Research of the State Key Laboratory of Agrobiotechnology and School of Life Sciences, The Chinese University of Hong Kong, Shatin, China.
Front Plant Sci. 2021 Mar 17;12:644408. doi: 10.3389/fpls.2021.644408. eCollection 2021.
Salinity is a major environmental factor that constrains soybean yield and grain quality. Given our past observations using the salt-sensitive soybean ( [L.] Merr.) accession C08 on its early responses to salinity and salt-induced transcriptomic modifications, the aim of this study was to assess the lipid profile changes in this cultivar before and after short-term salt stress, and to explore the adaptive mechanisms underpinning lipid homeostasis. To this end, lipid profiling and proteomic analyses were performed on the leaves of soybean seedlings subjected to salt treatment for 0, 0.5, 1, and 2 h. Our results revealed that short-term salt stress caused dynamic lipid alterations resulting in recycling for both galactolipids and phospholipids. A comprehensive understanding of membrane lipid adaption following salt treatment was achieved by combining time-dependent lipidomic and proteomic data. Proteins involved in phosphoinositide synthesis and turnover were upregulated at the onset of salt treatment. Salinity-induced lipid recycling was shown to enhance jasmonic acid and phosphatidylinositol biosyntheses. Our study demonstrated that salt stress resulted in a remodeling of membrane lipid composition and an alteration in membrane lipids associated with lipid signaling and metabolism in C08 leaves.
盐度是限制大豆产量和籽粒品质的主要环境因素。基于我们过去使用盐敏感大豆（[L.] Merr.）品种C08对盐度的早期响应及其盐诱导转录组修饰的观察，本研究旨在评估该品种在短期盐胁迫前后的脂质谱变化，并探索脂质稳态的适应性机制。为此，对经0、0.5、1和2小时盐处理的大豆幼苗叶片进行了脂质谱分析和蛋白质组学分析。我们的结果表明，短期盐胁迫导致脂质动态变化，导致半乳糖脂和磷脂的循环利用。通过结合时间依赖性脂质组学和蛋白质组学数据，全面了解了盐处理后的膜脂适应性。参与磷酸肌醇合成和周转的蛋白质在盐处理开始时上调。盐度诱导的脂质循环显示可增强茉莉酸和磷脂酰肌醇的生物合成。我们的研究表明，盐胁迫导致C08叶片中膜脂组成重塑以及与脂质信号传导和代谢相关的膜脂改变。
