Ghauri Sanaid, Raza Syed Qasim, Imran Muhammad, Saeed Shagufta, Rashid Muhammad, Naseer Rahat
Institute of Biochemistry and Biotechnology, The University of Veterinary and Animal Sciences, Lahore, Pakistan.
Dr. Panjwani Center for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan.
3 Biotech. 2021 Apr;11(4):167. doi: 10.1007/s13205-021-02717-8. Epub 2021 Mar 15.
Diabetes mellitus is a metabolic disorder of carbohydrate metabolism. The management of Diabetes mellitus with phytochemicals is hallmark of this research. Citrus species are known for their health benefits and are used as traditional food in South East Asia. The total phenolic content of peels was analyzed using different solvents, while Gallic acid was used as standard. Both ethanolic, aqueous extracts of peel showed good inhibitory activity against amylase (90.67%, 15.33%) and moderate against glucosidase (70.8%, 14.8%), respectively. Sixteen rats were randomly divided into four groups (G1, G2, G3, and G4); G1 is a negative control (water), G4 is a positive control (Acarbose), while other two are experimental groups like G2 (fed with 100 mL and 20 mg/mL in hypoglycemic and hyperglycemic trials) and G3 fed with 200 mL and 40 mg/mL in hypoglycemic and hyperglycemic trials. A significant effect of treatments and value of time was found in hyperglycemic rats. Ethanolic extract showed a significant reduction in blood glucose levels in hypoglycemic (overnight fasting) rats which was comparable to the positive control. These results suggest that peels can contribute as a useful food ingredient as a potential antihyperglycemic agent in managing type 2 diabetes mellitus. In future, peel will be a good candidate for pharmaceutical industry.
糖尿病是一种碳水化合物代谢的代谢紊乱疾病。用植物化学物质管理糖尿病是这项研究的标志。柑橘类物种因其对健康有益而闻名，并在东南亚被用作传统食物。使用不同溶剂分析了果皮的总酚含量，同时以没食子酸作为标准品。果皮的乙醇提取物和水提取物分别对淀粉酶表现出良好的抑制活性（90.67%，15.33%），对葡萄糖苷酶表现出中等抑制活性（70.8%，14.8%）。16只大鼠被随机分为四组（G1、G2、G3和G4）；G1为阴性对照（水），G4为阳性对照（阿卡波糖），另外两组为实验组，如G2（在降血糖和升血糖试验中分别喂食100 mL和20 mg/mL）和G3（在降血糖和升血糖试验中分别喂食200 mL和40 mg/mL）。在高血糖大鼠中发现了治疗和时间值的显著影响。乙醇提取物在低血糖（过夜禁食）大鼠中显示出血糖水平显著降低，与阳性对照相当。这些结果表明，果皮作为一种潜在的抗高血糖剂，可作为一种有用的食品成分用于管理2型糖尿病。未来，果皮将是制药行业的一个良好候选物。
