Biotechnology Research Center, Shandong Academy of Agricultural Sciences, Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Jinan 250100, People's Republic of China; College of Life Sciences, Shandong Normal University, Jinan 250014, People's Republic of China.
Cash Crop Research Institute, Guangxi Academy of Agricultural Sciences, 530007 Nanning, People's Republic of China.
Genomics. 2021 May;113(3):1579-1588. doi: 10.1016/j.ygeno.2021.03.030. Epub 2021 Apr 2.
The perennial ornamental peanut Arachis glabrata represents one of the most adaptable wild Arachis species. This study used PacBio combined with BGISEQ-500 RNA-seq technology to study the transcriptome and gene expression dynamics of A. glabrata. Of the total 109,747 unique transcripts obtained, >90,566 transcripts showed significant homology to known proteins and contained the complete coding sequence (CDS). RNA-seq revealed that 1229, 1039, 1671, 3923, 1521 and 1799 transcripts expressed specifically in the root, stem, leaf, flower, peg and pod, respectively. We also identified thousands of differentially expressed transcripts in response to drought, salt, cold and leaf spot disease. Furthermore, we identified 30 polyphenol oxidase encoding genes associated with the quality of forage, making A. glabrata suitable as a forage crop. Our findings presented the first transcriptome study of A. glabrata which will facilitate genetic and genomics studies and lays the groundwork for a deeper understanding of the A. glabrata genome.
多年生观赏花生 Arachis glabrata 是适应性最强的野生花生之一。本研究使用 PacBio 结合 BGISEQ-500 RNA-seq 技术研究了 A. glabrata 的转录组和基因表达动态。在总共获得的 109747 个独特转录本中，>90566 个转录本与已知蛋白质具有显著同源性，并包含完整的编码序列（CDS）。RNA-seq 显示，分别在根、茎、叶、花、刺和荚中特异性表达 1229、1039、1671、3923、1521 和 1799 个转录本。我们还鉴定了数千个对干旱、盐、冷和叶斑病有反应的差异表达转录本。此外，我们鉴定了 30 个与饲料质量相关的多酚氧化酶编码基因，使 A. glabrata 适合作为饲料作物。我们的研究结果首次对 A. glabrata 进行了转录组研究，这将促进遗传和基因组学研究，并为深入了解 A. glabrata 基因组奠定基础。
