Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA, USA.
Nat Methods. 2021 Apr;18(4):346-357. doi: 10.1038/s41592-021-01090-x. Epub 2021 Apr 7.
Directed evolution experiments are typically carried out using in vitro systems, bacteria, or yeast-even when the goal is to probe or modulate mammalian biology. Performing directed evolution in systems that do not match the intended mammalian environment severely constrains the scope and functionality of the targets that can be evolved. We review new platforms that are now making it possible to use the mammalian cell itself as the setting for directed evolution and present an overview of frontier challenges and high-impact targets for this approach.
定向进化实验通常在体外系统、细菌或酵母中进行，即使目标是探测或调节哺乳动物生物学。在与预期的哺乳动物环境不匹配的系统中进行定向进化严重限制了可进化目标的范围和功能。我们回顾了新的平台，这些平台现在使得可以将哺乳动物细胞本身用作定向进化的环境，并概述了该方法面临的前沿挑战和高影响力的目标。
