Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, 400067, People's Republic of China.
Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing, 400067, People's Republic of China.
Drug Des Devel Ther. 2021 Mar 31;15:1441-1457. doi: 10.2147/DDDT.S304885. eCollection 2021.
White tip silver needle, a slightly fermented white tea, is abundant in flavonoids, and it has great significance in terms of D-galactose/lipopolysaccharide-induced aging in mice.
We analyzed the antioxidant capacity of white tip silver needle flavonoids (WTSNF) in vitro, assessed the effects of WTSNF on organ indexes, pathological changes, liver function indexes, biochemical indicators, molecular biological indicators, and genes related to oxidation and inflammation.
Ultra-high performance liquid chromatography-tandem mass spectrometry results showed that WTSNF contained baicalin, kaempferol, kaempferide, quercetin, isorhamnetin, lespenephryl, and rutin. WTSNF showed strong scavenging ability for both 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) free radicals. Pathological analysis results showed that WTSNF reduced liver, kidney, and lung damage in mice with induced aging. In the serum and liver tissue, WTSNF effectively increased the antioxidant-related levels of superoxide dismutase, catalase, glutathione peroxidase, glutathione, and total antioxidant capacity and reduced the levels of aspartate aminotransferase, alanine aminotransferase, malondialdehyde and nitric oxide. WTSNF also reduced the inflammation-related levels of interleukin-6, interleukin-1 beta, tumor necrosis factor alpha (TNFα), and interferon gamma (IFN-γ) and increased the levels of interleukin-10 and interleukin-12. Furthermore, WTSNF upregulated the mRNA expression levels of cupro-zinc superoxide dismutase, manganese superoxide dismutase, catalase, glutathione peroxidase, interleukin-10, neuronal nitric oxide synthase, endothelial nitric oxide synthase, nuclear factor erythroid 2-related factor, heme oxygenase 1, NAD(P)H dehydrogenase [quinone] 1, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκB-α), and thioredoxin, while it downregulated the mRNA expression levels of interleukin-6, interleukin-18, interleukin-1 beta, TNFα, IFN-γ, inducible nitric oxide synthase, cyclooxygenase-2, and nuclear factor kappa-light chain-enhancer of activated B cells (NF-κB).
WTSNF is a high-quality natural product with antioxidative and anti-inflammatory properties that can inhibits D-galactose/lipopolysaccharide-induced aging in mice.
白毫银针是一种轻微发酵的白茶，富含黄酮类化合物，对 D-半乳糖/脂多糖诱导的衰老小鼠具有重要意义。
我们分析了白毫银针黄酮（WTSNF）的体外抗氧化能力，评估了 WTSNF 对器官指数、病理变化、肝功能指标、生化指标、分子生物学指标以及氧化和炎症相关基因的影响。
超高效液相色谱-串联质谱结果表明，WTSNF 含有黄芩苷、山奈酚、山奈素、槲皮素、异鼠李素、藜芦醇和芦丁。WTSNF 对 1,1-二苯基-2-苦基肼（DPPH）和 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）二铵盐（ABTS）自由基均具有较强的清除能力。病理分析结果表明，WTSNF 减轻了诱导衰老小鼠的肝、肾和肺损伤。在血清和肝组织中，WTSNF 有效提高了超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽和总抗氧化能力的抗氧化相关水平，降低了天冬氨酸转氨酶、丙氨酸转氨酶、丙二醛和一氧化氮的水平。WTSNF 还降低了白细胞介素-6、白细胞介素-1β、肿瘤坏死因子-α（TNFα）和干扰素-γ（IFN-γ）的炎症相关水平，增加了白细胞介素-10 和白细胞介素-12 的水平。此外，WTSNF 上调了铜锌超氧化物歧化酶、锰超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、白细胞介素-10、神经元型一氧化氮合酶、内皮型一氧化氮合酶、核因子红细胞 2 相关因子、血红素加氧酶 1、NAD（P）H 脱氢酶醌 1、核因子κ轻链增强子 B 细胞抑制剂、α（IκB-α）和硫氧还蛋白的 mRNA 表达水平，同时下调了白细胞介素-6、白细胞介素-18、白细胞介素-1β、TNFα、IFN-γ、诱导型一氧化氮合酶、环氧化酶-2 和核因子κB 的 mRNA 表达水平。
WTSNF 是一种具有抗氧化和抗炎特性的高质量天然产物，可抑制 D-半乳糖/脂多糖诱导的衰老小鼠衰老。
