Separation Science and Metabolomics Laboratory, Murdoch University, Murdoch, WA, Australia.
School of Biomedical Science, University of Western Australia, Crawley, WA, Australia.
Respir Res. 2021 Apr 9;22(1):105. doi: 10.1186/s12931-021-01682-3.
Idiopathic pulmonary fibrosis (IPF) is a chronic interstitial lung disease characterized by fibrosis and progressive loss of lung function. The pathophysiological pathways involved in IPF are not well understood. Abnormal lipid metabolism has been described in various other chronic lung diseases including asthma and chronic obstructive pulmonary disease (COPD). However, its potential role in IPF pathogenesis remains unclear.
In this study, we used ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS) to characterize lipid changes in plasma derived from IPF patients with stable and progressive disease. We further applied a data-independent acquisition (DIA) technique called SONAR, to improve the specificity of lipid identification.
Statistical modelling showed variable discrimination between the stable and progressive subjects, revealing differences in the detection of triglycerides (TG) and phosphatidylcholines (PC) between progressors and stable IPF groups, which was further confirmed by mass spectrometry imaging (MSI) in IPF tissue.
This is the first study to characterise lipid metabolism between stable and progressive IPF, with results suggesting disparities in the circulating lipidome with disease progression.
特发性肺纤维化（IPF）是一种以纤维化和进行性肺功能丧失为特征的慢性间质性肺疾病。IPF 涉及的病理生理途径尚不清楚。在包括哮喘和慢性阻塞性肺疾病（COPD）在内的各种其他慢性肺部疾病中，已经描述了异常的脂质代谢。然而，其在 IPF 发病机制中的潜在作用仍不清楚。
在这项研究中，我们使用超高效液相色谱-四极杆飞行时间质谱（UPLC-QTOF-MS）来描述来自 IPF 患者稳定和进行性疾病的血浆中的脂质变化。我们进一步应用了一种称为 SONAR 的非依赖性数据获取（DIA）技术，以提高脂质鉴定的特异性。
统计建模显示稳定和进行性受试者之间存在可变性区分，揭示了进展者和稳定 IPF 组之间甘油三酯（TG）和磷脂酰胆碱（PC）检测的差异，这在 IPF 组织中的质谱成像（MSI）中得到了进一步证实。
这是第一项描述稳定和进行性 IPF 之间脂质代谢的研究，结果表明随着疾病的进展，循环脂质组存在差异。
