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葡萄糖氧化酶:应用、来源与重组生产。

Glucose oxidase: Applications, sources, and recombinant production.

机构信息

Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.

出版信息

Biotechnol Appl Biochem. 2022 Jun;69(3):939-950. doi: 10.1002/bab.2165. Epub 2021 Apr 15.

Abstract

Glucose oxidase is a subset of oxidoreductase enzymes that catalyzes the transfer of electrons from an oxidant to a reductant. Glucose oxidases use oxygen as an external electron acceptor that releases hydrogen peroxide (H O ). Glucose oxidase has many applications in commercial processes, including improving the color and taste, increasing the persistence of food materials, removing the glucose from the dried egg, and eliminating the oxygen from different juices and beverages. Moreover, glucose oxidase, along with catalase, is used in glucose testing kits (especially in biosensors) to detect and measure the presence of glucose in industrial and biological solutions (e.g., blood and urine specimens). Hence, glucose oxidase is a valuable enzyme in the industry and medical diagnostics. Therefore, evaluating the structure and function of glucose oxidase is crucial for modifying as well as improving its catalytic properties. Finding different sources of glucose oxidase is an effective way to find the type of enzyme with the desired catalysis. Besides, the recombinant production of glucose oxidase is the best approach to produce sufficient amounts of glucose oxidase for various uses. Accordingly, the study of various aspects of glucose oxidase in biotechnology and bioprocessing is crucial.

摘要

葡萄糖氧化酶是氧化还原酶中的一个亚类,它催化电子从氧化剂向还原剂的转移。葡萄糖氧化酶利用氧气作为外部电子受体,释放过氧化氢 (H₂O₂)。葡萄糖氧化酶在商业过程中有许多应用,包括改善颜色和味道、增加食品材料的保质期、从干蛋中去除葡萄糖,以及从各种果汁和饮料中去除氧气。此外,葡萄糖氧化酶与过氧化氢酶一起用于葡萄糖测试试剂盒(特别是在生物传感器中),以检测和测量工业和生物溶液(例如血液和尿液样本)中葡萄糖的存在。因此,葡萄糖氧化酶在工业和医学诊断中是一种有价值的酶。因此,评估葡萄糖氧化酶的结构和功能对于修饰和改善其催化特性至关重要。寻找不同来源的葡萄糖氧化酶是寻找具有所需催化作用的酶的有效方法。此外,重组生产葡萄糖氧化酶是生产各种用途所需的足够量葡萄糖氧化酶的最佳方法。因此,在生物技术和生物加工中研究葡萄糖氧化酶的各个方面至关重要。

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