Pas Mathilde L, Vanneste Kevin, Bokma Jade, Van Driessche Laura, De Keersmaecker Sigrid C J, Roosens Nancy H, Haesebrouck Freddy, Boyen Filip, Pardon Bart
Department of Large Animal Internal Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium.
Sciensano, Transversal Activities in Applied Genomics, Brussels, Belgium.
Front Vet Sci. 2021 Mar 26;8:631716. doi: 10.3389/fvets.2021.631716. eCollection 2021.
Sepsis is a frequent life-threatening condition in young calves, requiring rapid broad spectrum and bactericidal therapy to maximize survival chances. Few studies have identified and characterized bacteria involved in sepsis in calves. This report demonstrates the involvement of a multidrug resistant , an emerging pathogen in human medicine, in a calf with suspected sepsis. was identified by MALDI-TOF MS from blood cultures of a critically ill calf. Susceptibility testing showed phenotypic resistance against ampicillin, gentamicin, potentiated sulphonamides, streptomycin, tetracyclines and intermediate susceptibility for enrofloxacin. Whole genome sequencing confirmed identification as and the multidrug resistant character of the isolate. Antimicrobial resistance genes acting against aminoglycosides, beta-lactam antibiotics, fosfomycin, quinolones, sulphonamides, trimethoprim and tetracyclines were found. The calf recovered after empirical parenteral therapy with enrofloxacin and sodium penicillin for seven days. Ancillary therapy consisted of fluid therapy, ketoprofen and doxapram hydrochloride. To the authors' knowledge, this is the first report characterizing a multidrug resistant isolate from blood culture in cattle. It is currently unknown whether animals and farms may act as reservoirs for multidrug resistant strains.
脓毒症是犊牛常见的危及生命的病症，需要迅速进行广谱杀菌治疗以最大程度提高存活几率。很少有研究鉴定并描述犊牛脓毒症相关的细菌。本报告证实了一种耐多药菌（一种在人类医学中出现的病原体）与一头疑似脓毒症的犊牛有关。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）从一头危重病犊牛的血培养物中鉴定出了该菌。药敏试验显示其对氨苄西林、庆大霉素、增效磺胺、链霉素、四环素呈表型耐药，对恩诺沙星呈中度敏感。全基因组测序证实了该菌的鉴定结果及其耐多药特性。发现了针对氨基糖苷类、β-内酰胺类抗生素、磷霉素、喹诺酮类、磺胺类、甲氧苄啶和四环素类药物的抗菌耐药基因。经恩诺沙星和青霉素钠经验性非肠道治疗7天后，犊牛康复。辅助治疗包括液体疗法、酮洛芬和盐酸多沙普仑。据作者所知，这是第一份描述从牛血培养物中分离出耐多药菌的报告。目前尚不清楚动物和养殖场是否可能成为耐多药菌菌株的储存宿主。
