College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China.
Zhejiang Provincial Key Laboratory of Plant Evolutionary Ecology and Conservation, Taizhou University, Taizhou 318000, China.
Int J Mol Sci. 2020 Feb 17;21(4):1337. doi: 10.3390/ijms21041337.
To assess changes of metabolite content and regulation mechanism of the phenolic acid biosynthesis pathway at different developmental stages of leaves, this study performed a combined metabolome and transcriptome analysis of Cyclocarya paliurus leaves at different developmental stages. Metabolite and transcript profiling were conducted by ultra-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometer and high-throughput RNA sequencing, respectively. Transcriptome identification showed that 58 genes were involved in the biosynthesis of phenolic acid. Among them, 10 differentially expressed genes were detected between every two developmental stages. Identification and quantification of metabolites indicated that 14 metabolites were located in the phenolic acid biosynthetic pathway. Among them, eight differentially accumulated metabolites were detected between every two developmental stages. Association analysis between metabolome and transcriptome showed that six differentially expressed structural genes were significantly positively correlated with metabolite accumulation and showed similar expression trends. A total of 128 transcription factors were identified that may be involved in the regulation of phenolic acid biosynthesis; these include 12 MYBs and 10 basic helix-loop-helix (bHLH) transcription factors. A regulatory network of the phenolic acid biosynthesis was established to visualize differentially expressed candidate genes that are involved in the accumulation of metabolites with significant differences. The results of this study contribute to the further understanding of phenolic acid biosynthesis during the development of leaves of C. paliurus.
为了评估不同发育阶段的叶片中酚酸生物合成途径的代谢物含量变化和调控机制，本研究对不同发育阶段的青钱柳叶进行了代谢组学和转录组学联合分析。代谢组学和转录组学分别通过超高效液相色谱-四极杆飞行时间串联质谱和高通量 RNA 测序进行。转录组鉴定表明，58 个基因参与了酚酸的生物合成。其中，在两个发育阶段之间检测到 10 个差异表达基因。代谢物的鉴定和定量表明，在两个发育阶段之间检测到 14 个代谢物位于酚酸生物合成途径中。其中，在两个发育阶段之间检测到 8 个差异积累代谢物。代谢组学和转录组学之间的关联分析表明，6 个差异表达的结构基因与代谢物积累呈显著正相关，且表达趋势相似。共鉴定到 128 个可能参与酚酸生物合成调控的转录因子；其中包括 12 个 MYB 和 10 个碱性螺旋-环-螺旋(bHLH)转录因子。建立了酚酸生物合成的调控网络，可视化了差异表达的候选基因，这些候选基因参与了具有显著差异的代谢物的积累。本研究的结果有助于进一步了解青钱柳叶发育过程中酚酸的生物合成。
