Khani Samira, Abdollahi Maasoume, Khalaj Azam, Heidari Hamid, Zohali Somaye
Neuroscience Research Center, Qom University of Medical Sciences, Qom, Iran.
Department of Anatomical Sciences, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran.
Int J Reprod Biomed. 2021 Mar 21;19(3):271-282. doi: 10.18502/ijrm.v19i3.8575. eCollection 2021 Mar.
Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders among women.
The aim of this study was to investigate the therapeutic effect of hydroalcoholic extract of () seed as a plant, the consumption of which has been recommended in Islam, on dehydroepiandrosterone (DHEA)-induced PCOS rats.
This experimental study was carried out on 36 Wistar female rats (3 wk, 60 10 gr). Then rats were divided into 6 groups (n = 6/each): control; PCOS-induced (DHEA 60 mg/kg/sc); PCOS+ Metformine (30 mg/kg); and three experimental groups receiving DHEA + hydroalcoholic extract of seeds in doses of 50, 100 and 200 mg/kg, respectively. Blood samples were taken for the evaluation of sexual hormones, oxidative stress, glucose, and insulin after 30 days of treatment. Ovarian tissue was used for histopathological study.
The serum levels of luteinizing hormone, testosterone, glucose, insulin resistance, malondialdehyde, and insulin (p 0.001) and estrogen increased while the levels of progesterone (p = 0.01) and antioxidant enzymes in the PCOS group decreased (p 0.001).
The administration of the extract to the PCOS rats resulted in remarkable changes in the serumic factors relative to the PCOS group. In addition, the extract improved the structure of the ovarian tissue in the PCOS rat. The histopathological results which are in accordance with biochemical findings imply that seed could be useful in the treatment of PCOS, the higher doses of the extract being more effective.
多囊卵巢综合征（PCOS）是女性中最常见的内分泌疾病之一。
本研究旨在探讨在伊斯兰教中被推荐食用的一种植物（此处原文括号内植物名称缺失）种子的水醇提取物对脱氢表雄酮（DHEA）诱导的PCOS大鼠的治疗效果。
本实验研究对36只Wistar雌性大鼠（3周龄，60±10克）进行。然后将大鼠分为6组（每组n = 6）：对照组；PCOS诱导组（DHEA 60毫克/千克，皮下注射）；PCOS +二甲双胍组（30毫克/千克）；以及三个分别接受50、100和200毫克/千克剂量的DHEA +该种子水醇提取物的实验组。治疗30天后采集血样以评估性激素、氧化应激、葡萄糖和胰岛素水平。卵巢组织用于组织病理学研究。
PCOS组中促黄体生成素、睾酮、葡萄糖、胰岛素抵抗、丙二醛和胰岛素的血清水平升高（p < 0.001），雌激素水平升高，而孕酮水平降低（p = 0.01），抗氧化酶水平降低（p < 0.001）。
与PCOS组相比，给PCOS大鼠施用该提取物导致血清因子发生显著变化。此外，该提取物改善了PCOS大鼠卵巢组织的结构。与生化结果一致的组织病理学结果表明，该种子可能对治疗PCOS有用，提取物剂量越高效果越明显。
