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对暴露于含铬和镍烟雾的电焊工淋巴细胞中DNA链断裂和交联频率以及姐妹染色单体交换情况的调查。

Investigations of the frequency of DNA strand breakage and cross-linking and of sister chromatid exchange in the lymphocytes of electric welders exposed to chromium- and nickel-containing fumes.

作者信息

Popp W, Vahrenholz C, Schmieding W, Krewet E, Norpoth K

机构信息

Institute for Hygiene and Industrial Medicine, University of Essen (GHS), Federal Republic of Germany.

出版信息

Int Arch Occup Environ Health. 1991;63(2):115-20. doi: 10.1007/BF00379074.

DOI:10.1007/BF00379074
PMID:1889880
Abstract

A total of 39 electric welders exposed to chromium and nickel were compared with 18 controls standardized for age, smoking habits and sex with respect to the frequency of sister chromatid exchange (SCE) and of DNA strand breakage and cross-linking (measured by the method of alkaline filter elution) in their blood lymphocytes. A significant correlation was found between the frequency of SCE and of individual DNA strand breakage and the concentration of chromium in the urine. Less DNA from the welders than from the control group was eluted through the two filter types used (polycarbonate and polyvinylidene fluoride filters). This must be interpreted as resulting from the presence of DNA-protein cross-links, which has the secondary effect of leading to a relative reduction in the measurable frequency of strand breakage amongst the welders. The present results are in good agreement with in vitro and in vivo investigations that confirm the importance of DNA-protein cross-links for the carcinogenic effect of chromium.

摘要

共对39名接触铬和镍的电焊工与18名在年龄、吸烟习惯和性别方面进行了标准化匹配的对照组人员进行了比较，检测了他们血液淋巴细胞中的姐妹染色单体交换（SCE）频率以及DNA链断裂和交联情况（采用碱性滤膜洗脱法测量）。发现SCE频率和个体DNA链断裂频率与尿中铬浓度之间存在显著相关性。与对照组相比，电焊工的DNA通过两种使用的滤膜（聚碳酸酯滤膜和聚偏二氟乙烯滤膜）洗脱出来的量更少。这必定是由于存在DNA-蛋白质交联所致，其附带效应是导致电焊工中可测量的链断裂频率相对降低。目前的结果与体外和体内研究结果高度一致，这些研究证实了DNA-蛋白质交联对铬致癌作用的重要性。

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DNA-protein cross-links as a biomarker of Cr(VI) exposure.DNA-蛋白质交联作为六价铬暴露的生物标志物。

本文引用的文献

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Nickel carbonate induces DNA-protein crosslinks and DNA strand breaks in rat kidney.
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DNA-protein cross-linking by chromium salts.铬盐导致的DNA-蛋白质交联
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Genotoxic and cytotoxic effects of 4-aroyl-1-nitrosohydrazine-carboxamides on O6-alkylguanine-DNA alkyltransferase-positive and -negative human cell lines.4-芳酰基-1-亚硝基肼甲酰胺对O6-烷基鸟嘌呤-DNA烷基转移酶阳性和阴性人细胞系的遗传毒性和细胞毒性作用。
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DNA-protein cross-links and sister chromatid exchange frequencies in lymphocytes and hydroxyethyl mercapturic acid in urine of ethylene oxide-exposed hospital workers.环氧乙烷暴露的医院工作人员淋巴细胞中的DNA-蛋白质交联和姐妹染色单体交换频率以及尿液中的羟乙基硫醚酸
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The carcinogen chromate causes DNA damage and inhibits drug-mediated induction of porphyrin accumulation and glucuronidation in chick embryo hepatocytes.致癌物铬酸盐会导致DNA损伤，并抑制药物介导的雏鸡胚胎肝细胞中卟啉积累和葡萄糖醛酸化的诱导。
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Chromium(VI)-induced DNA lesions and chromium distribution in rat kidney, liver, and lung.六价铬诱导的大鼠肾脏、肝脏和肺中的DNA损伤及铬分布。
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No cytogenetic effects in lymphocytes of stainless steel welders.不锈钢焊工淋巴细胞无细胞遗传学效应。
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Increased incidence of chromosomal aberrations in peripheral lymphocytes of retired nickel workers.退休镍工人外周血淋巴细胞染色体畸变发生率增加。
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