Department of Animal Science and Biotechnology and College of Ecology and Environmental Science, Kyungpook National University, Sangju, Republic of Korea.
Department of Horse/Companion and Wild Animals, College of Ecology and Environmental Science, Kyungpook National University, Sangju, Republic of Korea.
Vector Borne Zoonotic Dis. 2021 Jul;21(7):502-508. doi: 10.1089/vbz.2020.2764. Epub 2021 Apr 12.
is an obligate intracellular zoonotic bacterium with a global distribution. This study was conducted to investigate the prevalence of in different animals and to assess the potential role of these species as reservoirs of infection and transmission to humans. A total of 592 blood samples (105 beef cattle, 61 dairy cattle, 110 Korean native goats, 83 Boer goats, and 233 horses) were collected in the Republic of Korea (ROK). The DNA was detected from blood samples using the transposon-like repetitive region () by PCR method. The results showed that 22.7% of the Korean-native goats, 16.4% of the dairy cattle, 15.2% of the beef cattle, 6.0% of the Boer goats, and 5.2% of the horses were positive for . Significant differences were found between the animal species. The univariable binary logistic regression analysis revealed that the risk of contracting C. was significantly high by 5.4-fold in Korean-native goats (95% confidence interval [CI]: 2.60%-11.27%, = 0.000), 3.6-fold in dairy cattle (95% CI: 1.48%-8.82%, = 0.005), and 3.3-fold in beef cattle (95% CI: 1.51%-7.28%, = 0.003) compared with horses. A phylogenetic tree based on the gene revealed that our sequences had 92.2%-99.9% similarity and were clustered with those detected in humans, cattle, goats, dogs, rodents, and ticks. circulating in the ROK exhibits genetic variation. To the best of our knowledge, this is the first study to identify DNA in a horse in the ROK. These results suggest that cattle, goats, and horses can be potential reservoirs for and play an important role in the transmission of infection. Further studies should assess the pathogenicity of circulating in the ROK.
是一种普遍存在于全球的专性细胞内人畜共患细菌。本研究旨在调查其在不同动物中的流行情况，并评估这些物种作为感染储主和传播给人类的潜在作用。本研究共采集了韩国（ROK）的 592 份血液样本（105 份牛肉牛、61 份奶牛、110 份韩国本地山羊、83 份波尔山羊和 233 匹马）。通过 PCR 方法，利用转座子样重复区（）检测血液样本中的 DNA。结果显示，22.7%的韩国本地山羊、16.4%的奶牛、15.2%的肉牛、6.0%的波尔山羊和 5.2%的马检测出 阳性。不同动物种类之间存在显著差异。单变量二项逻辑回归分析显示，韩国本地山羊（95%置信区间 [CI]：2.60%-11.27%， = 0.000）、奶牛（95% CI：1.48%-8.82%， = 0.005）和肉牛（95% CI：1.51%-7.28%， = 0.003）感染 的风险显著增加了 5.4 倍、3.6 倍和 3.3 倍，与马相比。基于 基因构建的系统发育树显示，我们的序列与在人类、牛、山羊、狗、啮齿动物和蜱中检测到的序列具有 92.2%-99.9%的相似性，并聚类在一起。在 ROK 循环的 表现出遗传变异。据我们所知，这是首次在 ROK 马中鉴定出 DNA。这些结果表明，牛、山羊和马可能是 的潜在储主，并在感染传播中发挥重要作用。应进一步研究评估在 ROK 循环的 的致病性。
