Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, Ministry of Natural Resources of China, State Key Laboratory Breeding Base of Marine Genetic Resources, Fujian Key Laboratory of Marine Genetic Resources, Xiamen 361005, PR China.
School of Environmental Science, Harbin Institute of Technology, Harbin, PR China.
Int J Syst Evol Microbiol. 2021 Apr;71(4). doi: 10.1099/ijsem.0.004770.
A novel Gram-stain-negative, aerobic, gliding, rod-shaped and carotenoid-pigmented bacterium, designated A20-9, was isolated from a microbial consortium of polyethylene terephthalate enriched from a deep-sea sediment sample from the Western Pacific. Growth was observed at salinities of 1-8 %, at pH 6.5-8 and at temperatures of 10-40 °C. The results of phylogenetic analyses based on the genome indicated that A20-9 formed a monophyletic branch affiliated to the family , and the 16S rRNA gene sequences exhibited the maximum sequence similarity of 93.8 % with DSM 17368, followed by similarities of 90.4, 90.1 and 88.8 % with MCCC 1F01079, DSM 103558 and MCCC 1K02288, respectively. Its complete genome size was 4 035 598 bp, the genomic DNA G+C content was 43.2 mol%. Whole genome comparisons indicated that A20-9 and DSM 17368 shared 67.8 % average nucleotide identity, 62.7 % average amino acid identity value, 46.6% of conserved proteins and 17.8 % digital DNA-DNA hybridization identity. A20-9 contained MK-7 as the major respiratory quinone. Its major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phospatidylcholine; and the major fatty acids were iso-C (37.5 %), iso-C 3-OH (12.4 %), and summed feature 3 (Cω7 /C1ω6, 11.6 %). Combining the genotypic and phenotypic data, A20-9 could be distinguished from the members of other genera within the family and represents a novel genus, for which the name gen. nov., sp. nov. is proposed. The type strain is A20-9 (=MCCC 1A17358 =KCTC 72878).
一株新型革兰氏阴性、需氧、滑行、杆状、类胡萝卜素着色的细菌，被命名为 A20-9，从西太平洋深海沉积物样品中富集的聚对苯二甲酸乙二醇酯微生物共生体中分离得到。在盐度为 1-8%、pH 值为 6.5-8 和温度为 10-40°C 的条件下观察到生长。基于基因组的系统发育分析结果表明，A20-9 形成一个单系分支，隶属于 科，16S rRNA 基因序列与 DSM 17368 的相似度最高，为 93.8%，其次与 MCCC 1F01079、DSM 103558 和 MCCC 1K02288 的相似度分别为 90.4%、90.1%和 88.8%。其完整基因组大小为 4035598bp，基因组 DNA G+C 含量为 43.2mol%。全基因组比较表明，A20-9 和 DSM 17368 的平均核苷酸同一性为 67.8%，平均氨基酸同一性值为 62.7%，保守蛋白为 46.6%，数字 DNA-DNA 杂交同一性为 17.8%。A20-9 含有 MK-7 作为主要呼吸醌。其主要极性脂为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和磷脂酰胆碱；主要脂肪酸为 iso-C（37.5%）、iso-C 3-OH（12.4%）和总和特征 3（Cω7/C1ω6，11.6%）。结合基因型和表型数据，A20-9 可与 科内其他属的成员区分开来，代表一个新属，建议使用新属名 gen. nov.，sp. nov.。模式株为 A20-9（=MCCC 1A17358=KCTC 72878）。
