State Key Laboratory of Tree Genetics and Breeding, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, Guangdong, 510520, China.
State Key Laboratory of Tree Genetics and Breeding, Research Institute of Tropical Forestry, Chinese Academy of Forestry, Guangzhou, Guangdong, 510520, China; College of Biology and Food Engineering, Chongqing Three Gorges University, Wanzhou, Chongqing, 404100, China.
Plant Physiol Biochem. 2021 Jun;163:178-188. doi: 10.1016/j.plaphy.2021.03.057. Epub 2021 Apr 5.
Glycoside hydrolase family 1 (GH1) β-glucosidases (BGLUs) are encoded by a large number of genes and are involved in many developmental processes and stress responses in plants. Due to their importance in plant growth and development, genome-wide analyses have been conducted in the model plant species Arabidopsis thaliana, rice and maize but not in woody plant species, which have important economic and ecological value. In this study, we systematically analyzed Populus BGLUs (PtBGLUs) and demonstrated the involvement of several genes under stress conditions. Forty-four PtBGLUs were identified in Populus databases; these genes were located on 11 chromosomes, and the proteins of several PtBGLU genes were highly similar. More than 90% of PtBGLUs contain three conserved motifs. Collinearity results showed that 44 PtBGLU genes resulted from 12 tandem and 5 segmental duplication events. Phylogenetic analysis revealed that 128 BGLU genes from Populus trichocarpa, A. thaliana and Oryza sativa could be classified into 4 subgroups and subgroup Ⅱ and Ⅳ were differently having PtBGLUs and AtBGLUs. We further investigated whether several PtBGLUs responded to drought stress and ABA treatment, and the results showed that most of the selected BGLU genes were expressed in response to stress, which is consistent with previous studies involving rice and Arabidopsis homologous genes. Large numbers of stress-, hormone-, and development-related elements in the PtBGLU promoters suggest that BGLU genes may be involved in complex networks. Taken together, our results provide valuable information for an improved understanding of β-glucosidase function in woody plants.
糖苷水解酶家族 1（GH1）β-葡萄糖苷酶（BGLUs）由大量基因编码，参与植物的许多发育过程和应激反应。由于它们在植物生长和发育中的重要性，已在模式植物拟南芥、水稻和玉米中进行了全基因组分析，但在具有重要经济和生态价值的木本植物中尚未进行。在这项研究中，我们系统地分析了杨树 BGLUs（PtBGLUs），并证明了几个基因在应激条件下的参与。在杨树数据库中鉴定出 44 个 PtBGLU；这些基因位于 11 条染色体上，几个 PtBGLU 基因的蛋白质高度相似。超过 90%的 PtBGLU 含有三个保守基序。共线性结果表明，44 个 PtBGLU 基因来自 12 个串联和 5 个片段复制事件。系统发育分析表明，来自杨属、拟南芥和水稻的 128 个 BGLU 基因可分为 4 个亚组，亚组Ⅱ和Ⅳ分别具有不同的 PtBGLU 和 AtBGLU。我们进一步研究了几个 PtBGLU 是否对干旱胁迫和 ABA 处理有反应，结果表明，所选 BGLU 基因中的大多数在应激时表达，这与涉及水稻和拟南芥同源基因的先前研究一致。PtBGLU 启动子中大量的应激、激素和发育相关元件表明，BGLU 基因可能参与复杂的网络。总之，我们的研究结果为深入了解木质部植物中β-葡萄糖苷酶的功能提供了有价值的信息。
