Schmid Yannick R F, Bürgel Sebastian C, Misun Patrick M, Hierlemann Andreas, Frey Olivier
Department of Biosystems Science and Engineering, Bio Engineering Laboratory, ETH Zurich, Mattenstrasse 26, CH-4058 Basel, Switzerland.
ACS Sens. 2016 Jul 18;1(8):1028-1035. doi: 10.1021/acssensors.6b00272.
Electrical impedance spectroscopy (EIS) as a label free and noninvasive analysis method receives growing attention for monitoring three-dimensional tissue constructs. In this Article, we present the integration of an EIS readout function into the hanging-drop network platform, which has been designed for culturing microtissue spheroids in perfused multitissue configurations. Two pairs of microelectrodes have been implemented directly in the support of the hanging drops by using a small glass inlay inserted in the microfluidic structure. The pair of bigger electrodes is sensitive to the drop size and allows for drop size control over time. The pair of smaller electrodes is capable of monitoring, on the one hand, the size of microtissue spheroids to follow, for example, the growth of cancer microtissues, and, on the other hand, the beating of cardiac microtissues in situ. The presented results demonstrate the feasibility of an EIS readout within the framework of multifunctional hanging-drop networks.
电阻抗光谱法（EIS）作为一种无标记且非侵入性的分析方法，在监测三维组织构建体方面受到越来越多的关注。在本文中，我们展示了将EIS读出功能集成到悬滴网络平台中，该平台设计用于在灌注多组织配置中培养微组织球体。通过在微流体结构中插入一个小玻璃嵌体，在悬滴的支撑物中直接实现了两对微电极。较大的一对电极对液滴大小敏感，并允许随时间控制液滴大小。较小的一对电极一方面能够监测微组织球体的大小，例如跟踪癌症微组织的生长，另一方面能够监测心脏微组织在原位的跳动。所呈现的结果证明了在多功能悬滴网络框架内进行EIS读出的可行性。
