Bioinformatics Lab, National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, New Delhi, India.
PLoS One. 2021 Apr 14;16(4):e0247215. doi: 10.1371/journal.pone.0247215. eCollection 2021.
Long non-coding RNAs (lncRNAs) are defined as transcripts of greater than 200 nucleotides that play a crucial role in various cellular processes such as the development, differentiation and gene regulation across all eukaryotes, including plant cells. Since the last decade, there has been a significant rise in our understanding of lncRNA molecular functions in plants, resulting in an exponential increase in lncRNA transcripts, while these went unannounced from the major Angiosperm plant species despite the availability of large-scale high throughput sequencing data in public repositories. We, therefore, developed a user-friendly, open-access web interface, AlnC (Angiosperm lncRNA Catalogue) for the exploration of lncRNAs in diverse Angiosperm plant species using recent 1000 plant (1KP) trancriptomes data. The current version of AlnC offers 10,855,598 annotated lncRNA transcripts across 682 Angiosperm plant species encompassing 809 tissues. To improve the user interface, we added features for browsing, searching, and downloading lncRNA data, interactive graphs, and an online BLAST service. Additionally, each lncRNA record is annotated with possible small open reading frames (sORFs) to facilitate the study of peptides encoded within lncRNAs. With this user-friendly interface, we anticipate that AlnC will provide a rich source of lncRNAs for small-and large-scale studies in a variety of flowering plants, as well as aid in the improvement of key characteristics in relevance to their economic importance. Database URL: http://www.nipgr.ac.in/AlnC.
长链非编码 RNA(lncRNA)被定义为长度大于 200 个核苷酸的转录物,在真核生物(包括植物细胞)的各种细胞过程中发挥着至关重要的作用,如发育、分化和基因调控。在过去的十年中,我们对植物中 lncRNA 分子功能的理解有了显著提高,导致 lncRNA 转录本的数量呈指数级增长,尽管在公共存储库中有大规模高通量测序数据,但主要的被子植物物种中这些 lncRNA 并没有被报道。因此,我们开发了一个用户友好的、开放访问的网络界面 AlnC(被子植物 lncRNA 目录),用于使用最近的 1000 种植物(1KP)转录组数据探索不同被子植物物种中的 lncRNA。当前版本的 AlnC 提供了 10,855,598 个注释的 lncRNA 转录本,涵盖了 682 种被子植物物种的 809 种组织。为了改善用户界面,我们添加了浏览、搜索和下载 lncRNA 数据、交互式图形和在线 BLAST 服务的功能。此外,每个 lncRNA 记录都注释了可能的小开放阅读框(sORFs),以方便研究 lncRNA 内编码的肽。有了这个用户友好的界面,我们预计 AlnC 将为各种开花植物的小尺度和大尺度研究提供丰富的 lncRNA 资源,并有助于提高与经济重要性相关的关键特征。数据库网址:http://www.nipgr.ac.in/AlnC。