Jégousse Clara, Vannier Pauline, Groben René, Glöckner Frank Oliver, Marteinsson Viggó
School of Health Sciences, University of Iceland, Reykjavik, Iceland.
Microbiology Group, Matís ohf., Reykjavik, Iceland.
PeerJ. 2021 Apr 2;9:e11112. doi: 10.7717/peerj.11112. eCollection 2021.
Marine microorganisms contribute to the health of the global ocean by supporting the marine food web and regulating biogeochemical cycles. Assessing marine microbial diversity is a crucial step towards understanding the global ocean. The waters surrounding Iceland are a complex environment where relatively warm salty waters from the Atlantic cool down and sink down to the deep. Microbial studies in this area have focused on photosynthetic micro- and nanoplankton mainly using microscopy and chlorophyll measurements. However, the diversity and function of the bacterial and archaeal picoplankton remains unknown. Here, we used a co-assembly approach supported by a marine mock community to reconstruct metagenome-assembled genomes (MAGs) from 31 metagenomes from the sea surface and seafloor of four oceanographic sampling stations sampled between 2015 and 2018. The resulting 219 MAGs include 191 bacterial, 26 archaeal and two eukaryotic MAGs to bridge the gap in our current knowledge of the global marine microbiome.
海洋微生物通过支持海洋食物网和调节生物地球化学循环，对全球海洋的健康做出贡献。评估海洋微生物多样性是了解全球海洋的关键一步。冰岛周围的水域是一个复杂的环境，来自大西洋的相对温暖的咸水在这里冷却并下沉到深海。该地区的微生物研究主要集中在光合微型和纳米浮游生物上，主要使用显微镜和叶绿素测量方法。然而，细菌和古菌的微微型浮游生物的多样性和功能仍然未知。在这里，我们使用了一种由海洋模拟群落支持的共组装方法，从2015年至2018年期间在四个海洋学采样站的海面和海底采集的31个宏基因组中重建宏基因组组装基因组（MAG）。由此产生的219个MAG包括191个细菌、26个古菌和两个真核MAG，以填补我们目前对全球海洋微生物群落认识的空白。
