Department of Gynecology, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, 221000 Jiangsu, China.
Graduate School, Xuzhou Medical University, Xuzhou, Jiangsu 221000, China.
Biomed Res Int. 2021 Mar 30;2021:5594081. doi: 10.1155/2021/5594081. eCollection 2021.
The present study is aimed at providing systematic insight into the composition and expression of transfer RNA (tRNA) derivative transcription in high-grade serous ovarian cancer (HGSOC).
tRNA derivative expression profiles in three pairs of HGSOC and adjacent normal ovarian tissues were conducted by tRNA-derived small RNA fragment (tRF) and tRNA half (tiRNA) sequencing. The differentially expressed tRFs and tiRNAs between HGSOC and paired adjacent normal samples were screened. The targeted genes of differentially expressed tRFs and tiRNAs were screened. The Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) of target genes of tRFs and tiRNAs were analyzed.
There are a total of 20 significantly upregulated and 15 significantly downregulated tRFs and tiRNAs between the cancer group and the paracarcinoma group. The upregulated tRFs and tiRNAs are mucin-type O-glycan biosynthesis, glycosphingolipid biosynthesis, the glucagon signaling pathway, the AMPK signaling pathway, maturity-onset diabetes of the young, glycosphingolipid biosynthesis, the insulin signaling pathway, insulin resistance, leukocyte transendothelial migration, starch, and sucrose metabolism. The downregulated tRFs and tiRNAs are other glycan degradation, vitamin digestion and absorption, fatty acid elongation, and biosynthesis of unsaturated fatty acids.
There are significantly expressed tRFs and tiRNAs in HGSOC tissues, and these may provide potential diagnostic biomarkers and therapeutic targets for HGSOC.
本研究旨在系统地研究高级别浆液性卵巢癌（HGSOC）中转录 RNA（tRNA）衍生物转录的组成和表达。
通过 tRNA 衍生的小 RNA 片段（tRF）和 tRNA 半（tiRNA）测序，对 3 对 HGSOC 和相邻正常卵巢组织中的 tRNA 衍生物表达谱进行分析。筛选 HGSOC 与配对相邻正常样本之间差异表达的 tRF 和 tiRNA。筛选差异表达 tRF 和 tiRNA 的靶向基因。对 tRF 和 tiRNA 靶向基因进行基因本体论（GO）和京都基因与基因组百科全书（KEGG）分析。
在癌症组和癌旁组之间共有 20 个明显上调和 15 个明显下调的 tRF 和 tiRNA。上调的 tRF 和 tiRNA 与粘蛋白型 O-聚糖生物合成、糖脂生物合成、胰高血糖素信号通路、AMPK 信号通路、青少年发病的成年型糖尿病、糖脂生物合成、胰岛素信号通路、胰岛素抵抗、白细胞跨内皮迁移、淀粉和蔗糖代谢有关。下调的 tRF 和 tiRNA 与其他糖降解、维生素消化和吸收、脂肪酸延长和不饱和脂肪酸生物合成有关。
HGSOC 组织中存在明显表达的 tRF 和 tiRNA，它们可能为 HGSOC 提供潜在的诊断生物标志物和治疗靶点。
