免疫荧光显微镜揭示的分离鱼类黑素细胞的微管系统。
Microtubule system of isolated fish melanophores as revealed by immunofluorescence microscopy.
作者信息
Schliwa M, Osborn M, Weber K
出版信息
J Cell Biol. 1978 Jan;76(1):229-36. doi: 10.1083/jcb.76.1.229.
Abstract
The microtubule system of melanophores of the angelfish, Pterophyllum scalare, has been studied using antibodies prepared against purified porcine brain tubulin in indirect immunofluorescence microscopy. Melanophores were freed from the surrounding tissue components of isolated scales by mild enzymatic digestion and then allowed to settle on a glass cover slip. In both the dispersed and the aggregated states large numbers of fluorescent fibers are seen. The number and the astral arrangement of these fibers, which run from the central region to the periphery of the cell, are striking. The system of fluorescent fibers is replaced by diffuse fluorescence of moderate intensity after cold treatment, but is restored after rewarming the cells. Differences in the immunofluorescence profiles between cells with dispersed and aggregated pigment are discussed in relation to electron microscopic data available for this system.
摘要
利用针对纯化猪脑微管蛋白制备的抗体，通过间接免疫荧光显微镜技术对神仙鱼（Pterophyllum scalare）黑素细胞的微管系统进行了研究。通过温和的酶消化将黑素细胞从分离鳞片的周围组织成分中分离出来，然后使其沉降在玻璃盖玻片上。在分散状态和聚集状态下均可见大量荧光纤维。这些从细胞中心区域延伸至周边的纤维数量及其星状排列十分显著。经过冷处理后，荧光纤维系统被中等强度的弥漫性荧光所取代，但细胞复温后又恢复原状。结合该系统现有的电子显微镜数据，讨论了色素分散和聚集的细胞之间免疫荧光图谱的差异。
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