Center of Super-Diamond and Advanced Films (COSDAF) and Department of Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon, Hong Kong.
School of Science, Department of Chemistry, University of Management and Technology, Lahore, 54770, Pakistan.
Acta Biomater. 2021 Jul 1;128:262-276. doi: 10.1016/j.actbio.2021.04.014. Epub 2021 Apr 15.
Contact lenses are widely used for visual corrections. However, while wearing contact lenses, eyes typically face discomforts (itching, irritation, burning, etc.) due to foreign object sensation, lack of oxygen permeability, and tear film disruption as opposed to a lack of wetting agents. Eyes are also prone to ocular infections such as bacterial keratitis (BK) and fungal keratitis (FK) and inflammatory events such as contact lens-related acute red eye (CLARE), contact lens peripheral ulcer (CLPU), and infiltrative keratitis (IK) caused by pathogenic bacterial and fungal strains that contaminate contact lenses. Therefore, a good design of contact lenses should adequately address the need for wetting, the supply of antioxidants, and antifouling and antimicrobial efficacy. Here, we developed multifunctional gallic acid (GA), phytomolecules-coated zinc oxide nanoparticles (ZN), and phytomolecules-coated zinc oxide nanoparticles + gallic acid + tobramycin (ZGT)-coated contact lenses using a sonochemical technique. The coated contact lenses exhibited significant antibacterial (>log 5.60), antifungal, and antibiofilm performance against BK-causing multidrug resistant bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia. coli) and FK-related pathogenic fungal strains (Candida albicans, Aspergillus fumigatus, and Fusarium solani). The gallic acid, tobramycin, and phytomolecules-coated zinc oxide nanoparticles have different functionalities (-OH, -NH, -COOH, -COH, etc.) that enhanced wettability of the coated contact lenses as compared to that of uncoated ones and further enabled them to exhibit remarkable antifouling property by prohibiting adhesion of platelets and proteins. The coated contact lenses also showed significant antioxidant activity by scavenging DPPH and good cytocompatibility to human corneal epithelial cells and keratinocytes cell lines. STATEMENT OF SIGNIFICANCE: • Multifunctional coated lenses were developed with an efficient sonochemical approach. • Lens surface was modified with nanocoatings of ZnO nanoparticles, gallic acid, and tobramycin. • This synergistic combination endowed the lenses with remarkable antimicrobial activity. • Coated lenses also showed noteworthy antifouling and biofilm inhibition activities. • Coated lenses showed good antioxidant, biocompatibility, and wettability characteristics.
隐形眼镜被广泛用于视力矫正。然而，在佩戴隐形眼镜时，眼睛通常会因为异物感、缺乏氧气通透性和泪膜破裂而感到不适（瘙痒、刺激、灼热等），而不是缺乏润湿剂。眼睛还容易受到眼部感染，如细菌性角膜炎（BK）和真菌性角膜炎（FK），以及由污染隐形眼镜的致病细菌和真菌菌株引起的炎症性事件，如与隐形眼镜相关的急性红眼（CLARE）、隐形眼镜周边溃疡（CLPU）和浸润性角膜炎（IK）。因此，良好的隐形眼镜设计应充分满足润湿、抗氧化剂供应以及防污和抗菌效果的需求。在这里，我们使用超声化学技术开发了多功能没食子酸（GA）、植物分子涂层氧化锌纳米粒子（ZN）和植物分子涂层氧化锌纳米粒子+没食子酸+妥布霉素（ZGT）涂层隐形眼镜。涂层隐形眼镜对引起 BK 的多药耐药细菌（金黄色葡萄球菌、铜绿假单胞菌和大肠杆菌）和 FK 相关致病真菌菌株（白色念珠菌、烟曲霉和茄病镰刀菌）具有显著的抗菌（>log5.60）、抗真菌和抗生物膜性能。没食子酸、妥布霉素和植物分子涂层氧化锌纳米粒子具有不同的功能（-OH、-NH、-COOH、-COH 等），与未涂层的隐形眼镜相比，增强了涂层隐形眼镜的润湿性，并进一步使它们通过防止血小板和蛋白质的附着而表现出显著的防污性能。涂层隐形眼镜还通过清除 DPPH 显示出显著的抗氧化活性，并对人角膜上皮细胞和角质形成细胞系具有良好的细胞相容性。意义声明：• 采用高效超声化学方法开发了多功能涂层镜片。• 通过氧化锌纳米粒子、没食子酸和妥布霉素的纳米涂层对镜片表面进行了修饰。• 这种协同组合使镜片具有显著的抗菌活性。• 涂层镜片还表现出显著的防污和生物膜抑制活性。• 涂层镜片显示出良好的抗氧化、生物相容性和润湿性特征。
