Yang Tianli, Zhao Feng, Zhou Liuhua, Liu Jingyu, Xu Luwei, Dou Quanliang, Xu Zheng, Jia Ruipeng
Department of Urology, Nanjing First Hospital, Nanjing Medical University, Nanjing, China.
J Tissue Eng. 2021 Mar 31;12:20417314211001545. doi: 10.1177/20417314211001545. eCollection 2021 Jan-Dec.
Mesenchymal stem cells (MSCs) are a therapeutic tool for tissue engineering. However, many studies have recently shown that the therapeutic effects of MSCs are mediated by paracrine signaling and their secretory factors rather than their multidirectional differentiation ability. Exosomes isolated from the conditioned medium of MSCs are considered the main intercellular communication medium between MSCs and their target cells. Exosomes have been utilized in a novel cell-free therapy strategy that has attracted much attention. In this study, we evaluated the effects of a new cell-free tissue-engineered bladder (bladder acellular matrix combined with adipose-derived mesenchymal stem cell exosomes (AMEs)) in vivo and in vitro to prove that AMEs promoted tissue regeneration and functional recovery in a rat bladder replacement model.
间充质干细胞（MSCs）是组织工程的一种治疗工具。然而，最近许多研究表明，MSCs的治疗作用是由旁分泌信号及其分泌因子介导的，而非其多向分化能力。从MSCs条件培养基中分离出的外泌体被认为是MSCs与其靶细胞之间主要的细胞间通讯介质。外泌体已被应用于一种备受关注的新型无细胞治疗策略中。在本研究中，我们评估了一种新型无细胞组织工程膀胱（膀胱脱细胞基质联合脂肪来源间充质干细胞外泌体（AMEs））在体内和体外的效果，以证明AMEs在大鼠膀胱替代模型中促进了组织再生和功能恢复。
