Micalis Institute, INRAE, AgroParisTech, Université Paris-Saclay, 78350, Jouy-en-Josas, France; Université Paris-Est, Anses, Laboratory for Food Safety, 94700, Maisons-Alfort, France.
Micalis Institute, INRAE, AgroParisTech, Université Paris-Saclay, 78350, Jouy-en-Josas, France.
Food Microbiol. 2021 Sep;98:103759. doi: 10.1016/j.fm.2021.103759. Epub 2021 Mar 5.
B. cereus is a human pathogen associated with food poisoning leading to gastrointestinal disorders, as well as local and severe systemic infections. The pathogenic spectrum of B. cereus ranges from strains used as probiotics in humans to lethal highly toxic strains. In this study, we gathered a collection of 100 strains representative of the pathological diversity of B. cereus in humans, and characterized these strains for their cytotoxic potential towards human cells. We analyzed the correlation between cytotoxicity to epithelial and macrophage cells and the combination of 10 genes suspected to play a role during B. cereus virulence. We highlight genetic differences among isolates and studied correlations between genetic signature, cytotoxicity and strain pathological status. We hope that our findings will improve our understanding of the pathogenicity of B. cereus, thereby making it possible to improve both clinical diagnosis and food safety.
蜡样芽胞杆菌是一种与食物中毒有关的人类病原体，可导致胃肠道疾病，以及局部和严重的全身感染。蜡样芽胞杆菌的致病谱范围从用作人类益生菌的菌株到致命的高毒性菌株。在这项研究中，我们收集了 100 株代表人类蜡样芽胞杆菌病理多样性的菌株，并对这些菌株对人细胞的细胞毒性潜力进行了表征。我们分析了对上皮细胞和巨噬细胞的细胞毒性与 10 个疑似在蜡样芽胞杆菌毒力中发挥作用的基因组合之间的相关性。我们强调了分离株之间的遗传差异，并研究了遗传特征、细胞毒性和菌株病理状态之间的相关性。我们希望我们的发现将有助于提高我们对蜡样芽胞杆菌致病性的理解，从而有可能改善临床诊断和食品安全。
