Department of Electrical and Computer Engineering, University of California San Diego, La Jolla, CA, USA.
Neurobiology Section, Division of Biological Sciences, University of California San Diego, La Jolla, CA, USA.
Nat Neurosci. 2021 Jun;24(6):886-896. doi: 10.1038/s41593-021-00841-5. Epub 2021 Apr 19.
Many cognitive processes require communication between the neocortex and the hippocampus. However, coordination between large-scale cortical dynamics and hippocampal activity is not well understood, partially due to the difficulty in simultaneously recording from those regions. In the present study, we developed a flexible, insertable and transparent microelectrode array (Neuro-FITM) that enables investigation of cortical-hippocampal coordinations during hippocampal sharp-wave ripples (SWRs). Flexibility and transparency of Neuro-FITM allow simultaneous recordings of local field potentials and neural spiking from the hippocampus during wide-field calcium imaging. These experiments revealed that diverse cortical activity patterns accompanied SWRs and, in most cases, cortical activation preceded hippocampal SWRs. We demonstrated that, during SWRs, different hippocampal neural population activity was associated with distinct cortical activity patterns. These results suggest that hippocampus and large-scale cortical activity interact in a selective and diverse manner during SWRs underlying various cognitive functions. Our technology can be broadly applied to comprehensive investigations of interactions between the cortex and other subcortical structures.
许多认知过程需要大脑新皮层和海马体之间的通信。然而，由于难以同时从这些区域进行记录，因此对大规模皮质动力学和海马体活动之间的协调还了解甚少。在本研究中，我们开发了一种灵活、可插入和透明的微电极阵列（Neuro-FITM），可在海马体尖波涟漪（SWR）期间研究皮质-海马体协调。Neuro-FITM 的灵活性和透明性允许在大范围钙成像期间从海马体同时记录局部场电位和神经尖峰。这些实验表明，SWR 伴随着各种皮质活动模式，并且在大多数情况下，皮质激活先于海马体 SWR。我们证明，在 SWR 期间，不同的海马体神经群体活动与不同的皮质活动模式相关。这些结果表明，在各种认知功能下，海马体和大规模皮质活动以选择性和多样化的方式相互作用。我们的技术可以广泛应用于皮质与其他皮质下结构之间相互作用的综合研究。
