SARS-CoV-2 实验室标本的灭活。
Inactivation of SARS-CoV-2 Laboratory Specimens.
机构信息
1Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.
2Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.
出版信息
Am J Trop Med Hyg. 2021 Apr 20;104(6):2195-2198. doi: 10.4269/ajtmh.21-0229.
Abstract
The burden on diagnostic and research laboratories to provide reliable inactivation for biological specimens to allow for safe downstream processing is high during the coronavirus disease 2019 (COVID-19) pandemic. We provide safety data regarding commonly used chemical and physical inactivation procedures that verify their effectiveness against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).
摘要
在 2019 冠状病毒病（COVID-19）大流行期间，诊断和研究实验室为提供可靠的生物标本灭活以确保下游处理安全的负担很高。我们提供了有关常用化学和物理灭活程序的安全性数据，这些数据验证了它们对严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的有效性。
相似文献
1
Inactivation of SARS-CoV-2 Laboratory Specimens.SARS-CoV-2 实验室标本的灭活。
Am J Trop Med Hyg. 2021 Apr 20;104(6):2195-2198. doi: 10.4269/ajtmh.21-0229.
2
Influence of Different Inactivation Methods on Severe Acute Respiratory Syndrome Coronavirus 2 RNA Copy Number.不同灭活方法对严重急性呼吸综合征冠状病毒 2 核酸拷贝数的影响。
J Clin Microbiol. 2020 Jul 23;58(8). doi: 10.1128/JCM.00958-20.
3
Evaluation of stability and inactivation methods of SARS-CoV-2 in context of laboratory settings.评估 SARS-CoV-2 在实验室环境下的稳定性和失活动力学。
Med Microbiol Immunol. 2021 Aug;210(4):235-244. doi: 10.1007/s00430-021-00716-3. Epub 2021 Jul 1.
4
Potential False-Negative Nucleic Acid Testing Results for Severe Acute Respiratory Syndrome Coronavirus 2 from Thermal Inactivation of Samples with Low Viral Loads.低病毒载量样本热失活可导致严重急性呼吸综合征冠状病毒 2 的核酸检测结果出现潜在假阴性。
Clin Chem. 2020 Jun 1;66(6):794-801. doi: 10.1093/clinchem/hvaa091.
5
Assessment of inactivation procedures for SARS-CoV-2.SARS-CoV-2 灭活程序的评估。
J Gen Virol. 2021 Mar;102(3). doi: 10.1099/jgv.0.001539. Epub 2021 Jan 8.
6
Analysis of Inactivation of SARS-CoV-2 by Specimen Transport Media, Nucleic Acid Extraction Reagents, Detergents, and Fixatives.分析标本运输介质、核酸提取试剂、清洁剂和固定剂对 SARS-CoV-2 的灭活作用。
J Clin Microbiol. 2020 Oct 21;58(11). doi: 10.1128/JCM.01713-20.
7
In vitro inactivation of SARS-CoV-2 by commonly used disinfection products and methods.常用消毒产品和方法对 SARS-CoV-2 的体外灭活作用。
Sci Rep. 2021 Jan 28;11(1):2418. doi: 10.1038/s41598-021-82148-w.
8
Methods of Inactivation of SARS-CoV-2 for Downstream Biological Assays.用于下游生物学检测的 SARS-CoV-2 灭活方法。
J Infect Dis. 2020 Oct 1;222(9):1462-1467. doi: 10.1093/infdis/jiaa507.
9
Airborne Disinfection by Dry Fogging Efficiently Inactivates Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), Mycobacteria, and Bacterial Spores and Shows Limitations of Commercial Spore Carriers.干雾空气消毒能有效灭活严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）、分枝杆菌和细菌孢子，但对商用孢子载体有一定局限性。
Appl Environ Microbiol. 2021 Jan 15;87(3). doi: 10.1128/AEM.02019-20.
10
Selection and application of biological safety cabinets in diagnostic and research laboratories with special emphasis on COVID-19.诊断和研究实验室中生物安全柜的选择和应用，特别强调 COVID-19。
Rev Sci Instrum. 2021 Aug 1;92(8):081401. doi: 10.1063/5.0047716.
引用本文的文献
1
Jamaican fruit bat (Artibeus jamaicensis) insusceptibility to mucosal inoculation with SARS-CoV-2 Delta variant is not caused by receptor compatibility.牙买加果蝠（Artibeus jamaicensis）对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）德尔塔变种黏膜接种不敏感并非由受体兼容性所致。
Npj Viruses. 2024 Jul 16;2(1):26. doi: 10.1038/s44298-024-00037-1.
2
Augmentation of Omicron BA.1 pathogenicity in hamsters using intratracheal inoculation.通过气管内接种增强仓鼠体内奥密克戎BA.1的致病性。
Npj Viruses. 2024 Jan 16;2(1):3. doi: 10.1038/s44298-023-00012-2.
3
Evolution of Omicron lineage towards increased fitness in the upper respiratory tract in the absence of severe lung pathology.在无严重肺部病变的情况下，奥密克戎谱系在上呼吸道中向着适应性增强的方向进化。
Nat Commun. 2025 Jan 11;16(1):594. doi: 10.1038/s41467-025-55938-3.
4
Assessment of the impact of different SARS-CoV-2 inactivation operations on colistin sulfate plasma concentration results.评估不同的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）灭活操作对硫酸黏菌素血浆浓度结果的影响。
BMC Infect Dis. 2025 Jan 3;25(1):12. doi: 10.1186/s12879-024-10353-9.
5
Longitudinal analysis of the lung proteome reveals persistent repair months after mild to moderate COVID-19.对肺部蛋白质组的纵向分析揭示了轻度至中度新冠肺炎数月后持续的修复情况。
Cell Rep Med. 2024 Jul 16;5(7):101642. doi: 10.1016/j.xcrm.2024.101642. Epub 2024 Jul 8.
6
Preservation of scRNA-Seq Libraries Using Existing Inactivation Protocols.使用现有灭活方案保存单细胞RNA测序文库
Pathogens. 2024 Feb 13;13(2):167. doi: 10.3390/pathogens13020167.
7
Sex differences and individual variability in the captive Jamaican fruit bat (Artibeus jamaicensis) intestinal microbiome and metabolome.圈养牙买加果蝠（Artibeus jamaicensis）肠道微生物组和代谢组的性别差异和个体变异性。
Sci Rep. 2024 Feb 9;14(1):3381. doi: 10.1038/s41598-024-53645-5.
8
Effectiveness of TRIzol in Inactivating Animal Pathogens.TRIzol 对动物病原体的灭活效果。
Appl Biosaf. 2023 Dec 1;28(4):230-241. doi: 10.1089/apb.2022.0031. Epub 2023 Dec 5.
9
Low level of tonic interferon signalling is associated with enhanced susceptibility to SARS-CoV-2 variants of concern in human lung organoids.低水平的基础干扰素信号与人类肺类器官中对 SARS-CoV-2 关切变异株的易感性增强有关。
Emerg Microbes Infect. 2023 Dec;12(2):2276338. doi: 10.1080/22221751.2023.2276338. Epub 2023 Nov 16.
10
Histopathologic Characterization of Experimental Peracute SARS-CoV-2 Infection in the Syrian Hamster.叙利亚仓鼠实验性超急性严重急性呼吸综合征冠状病毒2感染的组织病理学特征
Vet Sci. 2023 Aug 23;10(9):536. doi: 10.3390/vetsci10090536.
Zotero 插件