SARS-CoV-2 实验室标本的灭活。

Inactivation of SARS-CoV-2 Laboratory Specimens.

机构信息

1Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.

2Rocky Mountain Veterinary Branch, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana.

出版信息

Am J Trop Med Hyg. 2021 Apr 20;104(6):2195-2198. doi: 10.4269/ajtmh.21-0229.

DOI:10.4269/ajtmh.21-0229

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8176508/

Abstract

The burden on diagnostic and research laboratories to provide reliable inactivation for biological specimens to allow for safe downstream processing is high during the coronavirus disease 2019 (COVID-19) pandemic. We provide safety data regarding commonly used chemical and physical inactivation procedures that verify their effectiveness against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).

摘要

在 2019 冠状病毒病（COVID-19）大流行期间，诊断和研究实验室为提供可靠的生物标本灭活以确保下游处理安全的负担很高。我们提供了有关常用化学和物理灭活程序的安全性数据，这些数据验证了它们对严重急性呼吸综合征冠状病毒 2（SARS-CoV-2）的有效性。

相似文献

1

Inactivation of SARS-CoV-2 Laboratory Specimens.

Am J Trop Med Hyg. 2021 Apr 20;104(6):2195-2198. doi: 10.4269/ajtmh.21-0229.

2

Influence of Different Inactivation Methods on Severe Acute Respiratory Syndrome Coronavirus 2 RNA Copy Number.

J Clin Microbiol. 2020 Jul 23;58(8). doi: 10.1128/JCM.00958-20.

3

Evaluation of stability and inactivation methods of SARS-CoV-2 in context of laboratory settings.

Med Microbiol Immunol. 2021 Aug;210(4):235-244. doi: 10.1007/s00430-021-00716-3. Epub 2021 Jul 1.

4

Potential False-Negative Nucleic Acid Testing Results for Severe Acute Respiratory Syndrome Coronavirus 2 from Thermal Inactivation of Samples with Low Viral Loads.

Clin Chem. 2020 Jun 1;66(6):794-801. doi: 10.1093/clinchem/hvaa091.

5

Assessment of inactivation procedures for SARS-CoV-2.

J Gen Virol. 2021 Mar;102(3). doi: 10.1099/jgv.0.001539. Epub 2021 Jan 8.

6

Analysis of Inactivation of SARS-CoV-2 by Specimen Transport Media, Nucleic Acid Extraction Reagents, Detergents, and Fixatives.

J Clin Microbiol. 2020 Oct 21;58(11). doi: 10.1128/JCM.01713-20.

7

In vitro inactivation of SARS-CoV-2 by commonly used disinfection products and methods.

Sci Rep. 2021 Jan 28;11(1):2418. doi: 10.1038/s41598-021-82148-w.

8

Methods of Inactivation of SARS-CoV-2 for Downstream Biological Assays.

J Infect Dis. 2020 Oct 1;222(9):1462-1467. doi: 10.1093/infdis/jiaa507.

9

Airborne Disinfection by Dry Fogging Efficiently Inactivates Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), Mycobacteria, and Bacterial Spores and Shows Limitations of Commercial Spore Carriers.

Appl Environ Microbiol. 2021 Jan 15;87(3). doi: 10.1128/AEM.02019-20.

10

Selection and application of biological safety cabinets in diagnostic and research laboratories with special emphasis on COVID-19.

Rev Sci Instrum. 2021 Aug 1;92(8):081401. doi: 10.1063/5.0047716.

引用本文的文献

1

Jamaican fruit bat (Artibeus jamaicensis) insusceptibility to mucosal inoculation with SARS-CoV-2 Delta variant is not caused by receptor compatibility.

Npj Viruses. 2024 Jul 16;2(1):26. doi: 10.1038/s44298-024-00037-1.

2

Augmentation of Omicron BA.1 pathogenicity in hamsters using intratracheal inoculation.

Npj Viruses. 2024 Jan 16;2(1):3. doi: 10.1038/s44298-023-00012-2.

3

Evolution of Omicron lineage towards increased fitness in the upper respiratory tract in the absence of severe lung pathology.

Nat Commun. 2025 Jan 11;16(1):594. doi: 10.1038/s41467-025-55938-3.

4

Assessment of the impact of different SARS-CoV-2 inactivation operations on colistin sulfate plasma concentration results.

BMC Infect Dis. 2025 Jan 3;25(1):12. doi: 10.1186/s12879-024-10353-9.

5

Longitudinal analysis of the lung proteome reveals persistent repair months after mild to moderate COVID-19.

Cell Rep Med. 2024 Jul 16;5(7):101642. doi: 10.1016/j.xcrm.2024.101642. Epub 2024 Jul 8.

6

Preservation of scRNA-Seq Libraries Using Existing Inactivation Protocols.

Pathogens. 2024 Feb 13;13(2):167. doi: 10.3390/pathogens13020167.

7

Sex differences and individual variability in the captive Jamaican fruit bat (Artibeus jamaicensis) intestinal microbiome and metabolome.

Sci Rep. 2024 Feb 9;14(1):3381. doi: 10.1038/s41598-024-53645-5.

8

Effectiveness of TRIzol in Inactivating Animal Pathogens.

Appl Biosaf. 2023 Dec 1;28(4):230-241. doi: 10.1089/apb.2022.0031. Epub 2023 Dec 5.

9

Low level of tonic interferon signalling is associated with enhanced susceptibility to SARS-CoV-2 variants of concern in human lung organoids.

Emerg Microbes Infect. 2023 Dec;12(2):2276338. doi: 10.1080/22221751.2023.2276338. Epub 2023 Nov 16.

10

Histopathologic Characterization of Experimental Peracute SARS-CoV-2 Infection in the Syrian Hamster.

Vet Sci. 2023 Aug 23;10(9):536. doi: 10.3390/vetsci10090536.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。