Ma Ying-Chao, Yang Xiu-Ling, Gu Jian-Jun
Department of Pharmacy, Second Hospital of Hebei Medical University, Shijiazhuang, 050000, China.
Department of Cardiac Surgery, Second Hospital of Hebei Medical University, No.215 of Heping West Road,Xinhua District, Shijiazhuang, 050000, China.
BMC Infect Dis. 2025 Jan 3;25(1):12. doi: 10.1186/s12879-024-10353-9.
To evaluate the effects of different SARS-CoV-2 inactivation methods on the blood concentration of colistin sulfate.
A colistin sulfate reference substance, a quality control plasma sample, and a clinically measured sample were transferred and heated in a 56 °C water batch for 30 min or irradiated under an ultraviolet (UV) lamp for 60 min to examine the stability of the reference solution and quality control plasma sample. Statistical analysis was conducted for the concentration of the clinically measured sample before and after inactivation with the intraclass correlation coefficient (ICC) method, the Passing-Bablok regression, and the Bland-Altman analysis.
The reference substance of colistin sulfate was unstable under UV inactivation. Under moist heat inactivation, the reference substance and quality control plasma sample of colistin sulfate were stable. The ICC of the concentration results of 27 clinical blood samples with and without moist heat inactivation was 0.995 (P < 0.001). The Passing-Bablok regression equation was Y = 0.01347 + 0.9861X (R = 0.994, n = 27). Finally, 96.30% (26/27) of test data was within the 95% limit of agreement. The results obtained with the three statistical analysis methods all showed a high correlation and agreement between the plasma concentration results of samples not inactivated and inactivated with moist heat inactivation.
The clinical plasma concentration test samples for colistin sulfate can be processed with the moist heat virus inactivation method to reduce the risk of virus infection of medical staff.
评估不同的严重急性呼吸综合征冠状病毒2（SARS-CoV-2）灭活方法对硫酸黏菌素血药浓度的影响。
将硫酸黏菌素对照品、质控血浆样本和临床检测样本转移至56℃水浴中加热30分钟或在紫外灯下照射60分钟，以检测对照溶液和质控血浆样本的稳定性。采用组内相关系数（ICC）法、Passing-Bablok回归法和Bland-Altman分析法对临床检测样本灭活前后的浓度进行统计分析。
硫酸黏菌素对照品在紫外线灭活条件下不稳定。在湿热灭活条件下，硫酸黏菌素对照品和质控血浆样本稳定。27份临床血样湿热灭活前后浓度结果的ICC为0.995（P＜0.001）。Passing-Bablok回归方程为Y = 0.01347 + 0.9861X（R = 0.994，n = 27）。最后，96.30%（26/27）的测试数据在95%一致性界限内。三种统计分析方法所得结果均显示未灭活样本与湿热灭活样本的血浆浓度结果之间具有高度相关性和一致性。
硫酸黏菌素临床血浆浓度检测样本可采用湿热病毒灭活方法处理，以降低医护人员病毒感染风险。
