Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Biomol Concepts. 2021 Apr 20;12(1):8-15. doi: 10.1515/bmc-2021-0002.
Doxorubicin (DOX) is an effective chemotherapeutic agent used for the treatment of various types of cancer. However, its poor solubility, undesirable side effects, and short half-life have remained a challenge. We used a formulation based on graphene oxide as an anticancer drug delivery system for DOX in MCF-7 breast cancer cells, to address these issues. release studies confirmed that the synthesized formulation has an improved release profile in acidic conditions (similar to the tumor microenvironment). Further studies, including MTT, uptake, and apoptosis assays were performed. The toxic effects of the nanocarrier on the kidney, heart and liver of healthy rats were also evaluated. We observed that the DOX-loaded carrier improved the cytotoxic effect of DOX on the breast cell line compared to free DOX. In summary, our results introduce the DOX-loaded carrier as a potential platform for targeting of cancer cells and suggest further studies are necessary to investigate its anti-cancer potential.
多柔比星（DOX）是一种有效的化疗药物，用于治疗多种类型的癌症。然而，其较差的溶解度、不理想的副作用和半衰期短仍然是一个挑战。我们使用基于氧化石墨烯的制剂作为 DOX 在 MCF-7 乳腺癌细胞中的抗癌药物递送系统，以解决这些问题。释放研究证实，合成制剂在酸性条件下（类似于肿瘤微环境）具有改善的释放特性。进一步的研究，包括 MTT、摄取和细胞凋亡测定也进行了。还评估了纳米载体对健康大鼠的肾脏、心脏和肝脏的毒性作用。我们观察到，与游离 DOX 相比，载 DOX 的载体提高了 DOX 对乳腺细胞系的细胞毒性作用。总之，我们的结果表明，载 DOX 的载体作为一种靶向癌细胞的潜在平台，并建议进一步研究其抗癌潜力。
