Department of Physiology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1946/1, 612 42 Brno, Czech Republic.
Department of Anatomy, Histology and Embryology, Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Palackého 1946/1, 612 42 Brno, Czech Republic.
Res Vet Sci. 2021 May;136:512-518. doi: 10.1016/j.rvsc.2021.04.003. Epub 2021 Apr 14.
Mast cells (MCs) and Kurloff cells (KCs) were detected in guinea pig ovaries in the follicular and luteal phases of the oestrous cycle. The samples of ovaries were fixed in Mota's basic lead acetate. Toluidine blue was used for detection of MCs and periodic acid-Schiff for detection of KCs. The percentage of KCs in a differential leukocyte count was determined in blood smears stained according to the Pappenheim method. Non-pregnant females with normal ovaries and with cystic rete ovarii were included in the study and the numbers of MCs and KCs were compared in these two groups and in follicular and luteal phases of the oestrous cycle. MCs' distribution in ovaries was different in the guinea pig in comparison to previously studied species: MCs were found exclusively in the superficial layers of cortical stroma and no significant difference was found between the number of MCs in the follicular and luteal phases, neither in normal ovaries, nor in ovaries with cystic rete ovarii. Significantly lower numbers of MCs were found in ovaries with cystic rete ovarii (P < 0.01) in contrast to normal ovaries. A significantly higher percentage of KCs in the peripheral blood was found in the follicular phase (P < 0.05), whereas no significant difference was found in relation to the presence of cystic rete ovarii. Interestingly, no KCs were found in the samples of ovaries (either in the follicular or luteal phase, and with or without cysts). Thus, the expected role of KCs in ovarian physiology or in the aetiology of the cystic rete ovarii can be excluded.
在发情周期的卵泡期和黄体期，豚鼠卵巢中检测到肥大细胞（MCs）和库尔洛夫细胞（KCs）。卵巢样本用 Mota 的基本醋酸铅固定。甲苯胺蓝用于检测 MCs，过碘酸-Schiff 用于检测 KCs。根据巴氏染色法对血液涂片进行染色，确定白细胞分类计数中 KCs 的百分比。本研究纳入了正常卵巢和囊性卵巢网的非妊娠雌性，比较了这两组和发情周期的卵泡期和黄体期的 MCs 和 KCs 数量。豚鼠卵巢中 MCs 的分布与之前研究的物种不同：MCs 仅存在于皮质基质的浅层，卵泡期和黄体期的 MCs 数量没有差异，无论是在正常卵巢中，还是在囊性卵巢网中都没有差异。与正常卵巢相比，囊性卵巢网中的 MCs 数量明显减少（P < 0.01）。在外周血中，卵泡期的 KCs 百分比明显更高（P < 0.05），而与囊性卵巢网的存在无关。有趣的是，卵巢样本中没有发现 KCs（无论是在卵泡期还是黄体期，有无囊肿）。因此，可以排除 KCs 在卵巢生理学或囊性卵巢网发病机制中的预期作用。
