Jörissen Jennifer, van den Broek Marianne F L, De Boeck Ilke, Van Beeck Wannes, Wittouck Stijn, Boudewyns An, Van de Heyning Paul, Topsakal Vedat, Van Rompaey Vincent, Wouters Ine, Van Heirstraeten Liesbet, Van Damme Pierre, Malhotra-Kumar Surbi, Theeten Heidi, Vanderveken Olivier M, Lebeer Sarah
Department of Bioscience Engineering, University of Antwerp, Antwerp, Belgium.
Department of Translational Neurosciences, Faculty of Medicine and Health Sciences, University of Antwerp, Wilrijk, Belgium.
mSystems. 2021 Apr 20;6(2):e00056-21. doi: 10.1128/mSystems.00056-21.
Chronic otitis media with effusion (OME) has been associated with a shift in microbiome composition and microbial interaction in the upper respiratory tract (URT). While most studies have focused on potential pathogens, this study aimed to find bacteria that could be protective against OME through a case-control microbiome study and characterization of isolates from healthy subjects. The URT and ear microbiome profiles of 70 chronic OME patients and 53 controls were compared by 16S rRNA amplicon sequencing. was the most frequent classic middle ear pathobiont. However, other taxa, especially , were also frequently detected in the ear canal of OME patients. Streptococci of the group and were more abundant in the nasopharynx of healthy controls than in OME patients. In addition to the microbiome analysis, 142 taxa were isolated from healthy individuals, and 79 isolates of 13 different species were tested for their pathobiont-inhibiting potential. Of these, isolates showed a superior capacity to inhibit the growth of , , , , , , and strains thus show potential as a probiotic for prevention or treatment of OME based on their overrepresentation in the healthy nasopharynx and their ability to inhibit the growth of respiratory pathobionts. (This study has been registered at ClinicalTrials.gov under registration no. NCT03109496.) The majority of probiotics marketed today target gastrointestinal health. This study searched for bacteria native to the human upper respiratory tract, with a beneficial potential for respiratory and middle ear health. Comparison of the microbiomes of children with chronic otitis media with effusion (OME) and of healthy controls identified as a health-associated and prevalent inhabitant of the human nasopharynx. However, beneficial potential should be assessed at strain level. Here, we also isolated specific strains from the healthy individuals in our study. These isolates showed a beneficial safety profile and efficacy potential to inhibit OME pathogens These properties will now have to be evaluated and confirmed in human clinical studies.
分泌性中耳炎（OME）与上呼吸道（URT）微生物群组成的改变及微生物相互作用有关。虽然大多数研究集中在潜在病原体上，但本研究旨在通过病例对照微生物群研究以及对健康受试者分离株的特性分析，寻找可能对OME具有保护作用的细菌。通过16S rRNA扩增子测序比较了70例慢性OME患者和53例对照的URT和耳部微生物群谱。 是最常见的经典中耳致病共生菌。然而，其他分类群，尤其是 ，在OME患者的耳道中也经常被检测到。健康对照的鼻咽部中 群链球菌和 比OME患者中更为丰富。除了微生物群分析外，还从健康个体中分离出142个分类群，并对13种不同 物种的79株分离株进行了致病共生菌抑制潜力测试。其中， 株分离物显示出对 、 、 、 、 、 和 菌株生长的卓越抑制能力，因此基于它们在健康鼻咽部中的高丰度以及抑制呼吸道致病共生菌生长的能力，显示出作为预防或治疗OME的益生菌的潜力。（本研究已在ClinicalTrials.gov注册，注册号为NCT03109496。）当今市场上的大多数益生菌针对胃肠道健康。本研究寻找人类上呼吸道原生的、对呼吸道和中耳健康具有有益潜力的细菌。对慢性分泌性中耳炎（OME）儿童和健康对照的微生物群进行比较，确定 是人类鼻咽部与健康相关且普遍存在的居民。然而，有益潜力应在菌株水平上进行评估。在这里，我们还从本研究中的健康个体中分离出特定的 菌株。这些分离物显示出有益的安全性和抑制OME病原体的功效潜力。这些特性现在必须在人体临床研究中进行评估和确认。
