山梨酸及山梨酸-亚硝酸盐在小鼠体内诱导的姐妹染色单体交换和微核形成

Sister chromatid exchanges and micronuclei formations induced by sorbic acid and sorbic acid-nitrite in vivo in mice.

作者信息

Mukherjee A, Giri A K, Talukder G, Sharma A

机构信息

Department of Botany, University of Calcutta, India.

出版信息

Toxicol Lett. 1988 Jul;42(1):47-53. doi: 10.1016/0378-4274(88)90101-4.

DOI:10.1016/0378-4274(88)90101-4

PMID:3388447

Abstract

The in vivo induction of sister chromatid exchanges and micronuclei formations by acute treatment with different concentrations of sorbic acid and by nitrite, individually and in combination, was studied in bone marrow cells of mice. A significant increase in the frequency of sister chromatid exchanges was only observed with the three higher concentrations of sorbic acid when compared to a distilled water control. Sodium nitrite produced a significant increase at all doses tested. A combination of half the concentration of sorbic acid and of sodium nitrite gave an additive effect over that of sorbic acid or sodium nitrite alone. In the micronucleus assay, the highest dose of sorbic acid (150 mg/kg body weight) produced a significant increase in micronuclei formations compared to the distilled water control. Sodium nitrite alone induced significant numbers of micronuclei at all concentrations tested when compared to the negative control. However, a combination of half the concentration of sorbic acid and of sodium nitrite gave synergistic effects which could possibly be ascribed to the formation of certain genotoxic compounds in vivo.

摘要

研究了不同浓度的山梨酸单独及与亚硝酸盐联合急性处理对小鼠骨髓细胞体内姐妹染色单体交换和微核形成的诱导作用。与蒸馏水对照相比，仅在三种较高浓度的山梨酸处理组中观察到姐妹染色单体交换频率显著增加。亚硝酸钠在所有测试剂量下均使姐妹染色单体交换频率显著增加。山梨酸浓度减半与亚硝酸钠联合使用时，其效果比单独使用山梨酸或亚硝酸钠具有相加作用。在微核试验中，与蒸馏水对照相比，山梨酸最高剂量（150mg/kg体重）使微核形成显著增加。与阴性对照相比，亚硝酸钠在所有测试浓度下均诱导产生大量微核。然而，山梨酸浓度减半与亚硝酸钠联合使用产生协同作用，这可能归因于体内某些遗传毒性化合物的形成。