Division of Crop Improvement, ICAR-Sugarcane Breeding Institute, Coimbatore, Tamil Nadu, 641007, India.
Plant Genetic Engineering Laboratory, Department of Biotechnology, Bharathiar University, Coimbatore, Tamil Nadu, 641041, India.
J Plant Res. 2021 Sep;134(5):1083-1094. doi: 10.1007/s10265-021-01300-9. Epub 2021 Apr 22.
The glyoxalase pathway is a check point to monitor the elevation of methylglyoxal (MG) level in plants and is mediated by glyoxalase I (Gly I) and glyoxalase II (Gly II) enzymes in the presence of glutathione. Recent studies established the presence of unique DJ-1/PfpI domain containing protein named glyoxalase III (Gly III) in prokaryotes, involved in the detoxification of MG into D-lactic acid through a single step process. In the present study, eleven transgenic sugarcane events overexpressing EaGly III were assessed for salinity stress (100 mM and 200 mM NaCl) tolerance. Lipid peroxidation as well as cell membrane injury remained very minimal in all the transgenic events indicating reduced oxidative damage. Transgenic events exhibited significantly higher plant water status, gas exchange parameters, chlorophyll, carotenoid, and proline content, total soluble sugars, SOD and POD activity compared to wild type (WT) under salinity stress. Histological studies by taking the cross section showed a highly stable root system in transgenic events upon exposure to salinity stress. Results of the present study indicate that transgenic sugarcane events overexpressing EaGly III performed well and exhibited improved salinity stress tolerance.
糖氧还蛋白途径是监测植物中甲基乙二醛 (MG) 水平升高的一个检查点，该途径由谷胱甘肽存在时的糖氧还酶 I (Gly I) 和糖氧还酶 II (Gly II) 酶介导。最近的研究在原核生物中建立了存在独特的 DJ-1/PfpI 结构域的蛋白，命名为糖氧还酶 III (Gly III)，它通过单一步骤过程将 MG 解毒成 D-乳酸。在本研究中，评估了 11 个过表达 EaGly III 的转基因甘蔗事件对盐胁迫（100 mM 和 200 mM NaCl）的耐受性。所有转基因事件中的脂质过氧化以及细胞膜损伤都非常小，表明氧化损伤减少。与野生型 (WT) 相比，转基因事件在盐胁迫下表现出更高的植物水势、气体交换参数、叶绿素、类胡萝卜素、脯氨酸含量、总可溶性糖、SOD 和 POD 活性。通过取横截面进行的组织学研究表明，在暴露于盐胁迫下，转基因事件具有高度稳定的根系。本研究的结果表明，过表达 EaGly III 的转基因甘蔗事件表现良好，并表现出提高的耐盐性。
