College of Pharmacy, Ningxia Medical University, 1160 Shengli Street, Yinchuan 750004 Ningxia, China.
College of Pharmacy, Ningxia Medical University, 1160 Shengli Street, Yinchuan 750004 Ningxia, China; Ningxia Engineering and Technology Research Center for Modernization of Regional Characteristic Traditional Chinese Medicine, Ningxia Medical University, Yinchuan, China; Key Laboratory of Hui Ethnic Medicine Modernisation, Ministry of Education, Ningxia Medical University, Yinchuan, China.
Int Immunopharmacol. 2021 Aug;97:107614. doi: 10.1016/j.intimp.2021.107614. Epub 2021 Apr 22.
Overexposure to ultraviolet B (UVB) rays can cause damage to the skin. Liquiritin has a variety of pharmacological effects, such as anti-inflammatory and antioxidant. In the present study, the effect of liquiritin on UVB irradiated rat skin was investigated. Results showed that UVB irradiation caused erythema and wrinkles on the skin surface, as well as thickening and loss of elasticity of the epidermis and a significant increase in the level of ROS in the skin tissue. At the same time, western blot detected an increase in nuclear factor kappa-B (NF-κB) and matrix metalloproteinases (MMPs) and Elisa also detected an increase in pro-inflammatory factors. Therefore, we hypothesized that UVB irradiation-induced damage is associated with inflammation. Interestingly, application of liquiritin to exposed skin of rats reduced the increase in ROS, pro-inflammatory factors, and MMPs caused by UVB irradiation and increased the levels of Sirtuin3 (SIRT3) and Collagen α1. In addition, after intraperitoneal injection of the SIRT3 inhibitor 3-TYP in rats, the protective effect of liquiritin against UVB damage was found to be diminished. These results suggested that promotion of SIRT3 with liquiritin inhibits UVB-induced production of pro-inflammatory mediators, possibly acting through the SIRT3/ROS/NF-κB pathway. In conclusion, this study suggests that liquiritin is an effective drug candidate for the prevention of UVB damage.
过度暴露于紫外线 B（UVB）射线会对皮肤造成损伤。甘草素具有多种药理作用，如抗炎和抗氧化。本研究探讨了甘草素对 UVB 照射大鼠皮肤的影响。结果表明，UVB 照射会导致皮肤表面出现红斑和皱纹，表皮增厚和弹性丧失，以及皮肤组织中 ROS 水平显著升高。同时，Western blot 检测到核因子 kappa-B（NF-κB）和基质金属蛋白酶（MMPs）的增加，Elisa 也检测到促炎因子的增加。因此，我们假设 UVB 照射引起的损伤与炎症有关。有趣的是，将甘草素应用于大鼠暴露的皮肤可减少 UVB 照射引起的 ROS、促炎因子和 MMPs 的增加，并增加 Sirtuin3（SIRT3）和 Collagen α1 的水平。此外，在大鼠腹腔内注射 SIRT3 抑制剂 3-TYP 后，发现甘草素对 UVB 损伤的保护作用减弱。这些结果表明，甘草素通过促进 SIRT3 抑制 UVB 诱导的促炎介质的产生，可能通过 SIRT3/ROS/NF-κB 途径发挥作用。总之，本研究表明，甘草素是预防 UVB 损伤的有效药物候选物。
