Department of Obstetrics and Gynecology, Hadassah Medical Center - Hebrew University of Jerusalem, Jerusalem, Israel.
The Casali Institute of Applied Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel.
Contraception. 2021 Sep;104(3):319-323. doi: 10.1016/j.contraception.2021.03.032. Epub 2021 Apr 21.
To determine if PF-88, a reverse thermo-responsive polymer designed to create a gel at body temperature and liquefy at a lower temperature (21°C) can reversibly occlude the fallopian tubes in rabbits.
Mature female New Zealand White rabbits underwent laparotomy and placement of 22-gage catheter into the lumen of the distil uterine horns for evaluation of tubal patency by fluoroscopy using radio opaque contrast and treatment with PF-88. In the Acute Phase group (n = 5) after PF-88 treatment we immediately cooled the serosal surface of the tube with ice for 90 seconds to liquify the gel then reassessed patency. In the Survival Phase groups, animals recovered from the initial surgery and then underwent a second procedure for evaluation of tubal occlusion and reversibility at 4 (n = 3), 14 (n = 2), and 28 (n = 3) weeks after the initial procedure. We compared the histologic appearance of the treated fallopian tubes to untreated controls (n = 3).
In the Acute Phase, we found all 10 fallopian tubes patent on initial evaluation, occluded following treatment with PF88, and patent following re-liquification by chilling. Animals in the Survival Group, all but one of the treated tubes appeared blocked at follow-up and patent following chilling. The treatment failure occurred in an animal in the 4-week group. Tubes treated with PF88 showed no histologic evidence of residual material or damage after removal of the polymer.
The PF-88 reverse thermo-responsive polymer demonstrated the ability to reversibly block fallopian tubes for up to 28 weeks.
The demonstration of reversible occlusion of the fallopian tube of rabbits using PF-88, a thermo-responsive reverse polymer, support additional studies to evaluate the potential of this polymer as a contraceptive in women.
确定 PF-88（一种设计用于在体温下形成凝胶并在较低温度（21°C）下液化的反向热响应聚合物）是否可以在兔子体内可逆地阻塞输卵管。
成熟的雌性新西兰白兔接受剖腹术，并将 22 号导管放置在子宫角的管腔内，通过透视使用不透射线的对比剂评估输卵管通畅性，并进行 PF-88 治疗。在急性阶段组（n=5）中，PF-88 治疗后，我们立即用冰冷却管的浆膜表面 90 秒，使凝胶液化，然后重新评估通畅性。在存活阶段组中，动物从初次手术中恢复过来，然后进行第二次手术，在初次手术后 4（n=3）、14（n=2）和 28（n=3）周时评估输卵管阻塞和可逆性。我们将处理过的输卵管的组织学外观与未经处理的对照（n=3）进行了比较。
在急性阶段，我们发现最初评估时所有 10 个输卵管都是通畅的，用 PF88 治疗后阻塞，用冷却再液化后又恢复通畅。存活组中的动物，除 1 只外，所有处理过的输卵管在随访时均显示阻塞，用冷却后又恢复通畅。治疗失败发生在 4 周组的一只动物中。PF88 处理过的输卵管在去除聚合物后没有残留物质或损伤的组织学证据。
PF-88 反向热响应聚合物证明能够在长达 28 周的时间内可逆地阻塞输卵管。
PF-88（一种热响应反向聚合物）在兔子输卵管中可逆阻塞的证明，支持进一步研究该聚合物作为女性避孕药的潜力。
