多色单分子 FRET 用于 DNA 和 RNA 过程。
Multicolor single-molecule FRET for DNA and RNA processes.
机构信息
Department of Biology, Johns Hopkins University, Baltimore, MD, USA; Department of Biophysics, Johns Hopkins University, Baltimore, MD, USA.
Department of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, MD, USA.
出版信息
Curr Opin Struct Biol. 2021 Oct;70:26-33. doi: 10.1016/j.sbi.2021.03.005. Epub 2021 Apr 21.
Abstract
Single-molecule fluorescence resonance energy transfer (smFRET) is a useful tool for observing the dynamics of protein-nucleic acid interactions. Although most smFRET measurements have used two fluorophores, multicolor smFRET measurements using more than two fluorophores offer more information about how protein-nucleic acid complexes dynamically move, assemble, and disassemble. Multicolor smFRET experiments include three or more fluorophores and at least one donor-acceptor pair. This review highlights how multicolor smFRET is being used to probe the dynamics of three different classes of biochemical processes-protein-DNA interactions, chromatin remodeling, and protein translation.
摘要
单分子荧光共振能量转移（smFRET）是观察蛋白质-核酸相互作用动力学的有用工具。尽管大多数 smFRET 测量都使用了两个荧光团，但使用三个或更多荧光团的多色 smFRET 测量提供了更多关于蛋白质-核酸复合物如何动态移动、组装和拆卸的信息。多色 smFRET 实验包括三个或更多的荧光团和至少一个供体-受体对。这篇综述强调了多色 smFRET 如何用于探测三种不同类型生化过程的动力学 - 蛋白质-DNA 相互作用、染色质重塑和蛋白质翻译。
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