Shangguan Huimin, Liu Qianwei, Wang Yuanjuan, Teng Zixuan, Tian Rumeng, Wu Tong, Yang Lei, Jiang Ling, Liu Xingjiang, Wei Liuhe
College of Ecology and Environment, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
International College, Zhengzhou University, Zhengzhou 450001, Henan Province, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2023 Dec 15;303:123256. doi: 10.1016/j.saa.2023.123256. Epub 2023 Aug 11.
Hypochlorous acid (HClO) is a reactive oxygen species and a relatively strong antibacterial substance in the immune defense system. The normal concentration of HClO in the human body is approximately 200 μM, and its high concentration can cause tissue damage and some diseases. Herein, a chromenoquinoline-based ratiometric fluorescent probe was developed to detect and quantify HClO. The developed Probe 1 exhibited the advantages of large Stokes shift (137 nm), high synthetic yield (84.7 %), simple synthesis method, short response time (<4 min), low detection limit (5.1 nM), and low toxicity. The probe was successfully validated in live cells and zebrafish.
次氯酸（HClO）是一种活性氧物种，是免疫防御系统中一种相对较强的抗菌物质。人体中HClO的正常浓度约为200μM，其高浓度会导致组织损伤和一些疾病。在此，开发了一种基于色烯喹啉的比率荧光探针来检测和定量HClO。所开发的探针1具有斯托克斯位移大（137nm）、合成产率高（84.7%）、合成方法简单、响应时间短（<4分钟）、检测限低（5.1 nM）和低毒性等优点。该探针已在活细胞和斑马鱼中成功验证。
