Abbas H K, Mirocha C J
Department of Plant Pathology, University of Minnesota, St. Paul 55108.
Appl Environ Microbiol. 1988 May;54(5):1268-74. doi: 10.1128/aem.54.5.1268-1274.1988.
An isolate of Fusarium oxysporum Schlecht, emend. Synd. et Hans. N17B isolated from a grassy area in Lakselv, Norway (Arctic region) produced a toxin in culture when grown on rice in the laboratory. This new toxin, which was given the trivial name of H-1 (indicating hemorrhagic factor), caused toxic effects in rats, including food refusal, weight loss, hemorrhage in the stomach, intestines, heart, and thymus, and finally death. The UV spectrum of H-1 showed 210, 254, and 292 nm as absorption maxima. The infrared spectrum showed carbonyl groups at 1,675 and 1,750 cm-1 and an ether group at 1,215 cm-1. H-1 does not fluoresce under short- or long-wavelength UV light and exists as fluffy, white crystals that turn yellow when subjected to basic reagents such as ammonium hydroxide or tetraethylenepentamine. Elemental and accurate mass determinations in both electron impact and positive chemical ionization indicate an empirical formula of C23H24O8. Its mass spectra (electron impact, chemical ionization, and fast atom bombardment [FAB]) show a molecular ion of 428 and major fragments at m/z+ 386, 368, 355, and 295. H-1 was found to be identical to the antibiotic called wortmannin which is produced by Penicillium wortmannii and Myrothecium roridum. This is the first report of the synthesis of wortmannin by species of the genus Fusarium.
从挪威拉克塞尔夫（北极地区）的一片草地分离得到的尖孢镰刀菌（Fusarium oxysporum Schlecht, emend. Synd. et Hans.）菌株N17B，在实验室条件下于大米培养基上培养时会产生一种毒素。这种新毒素被赋予了俗名H - 1（表示出血因子），它对大鼠具有毒性作用，包括拒食、体重减轻、胃、肠、心脏和胸腺出血，最终导致死亡。H - 1的紫外光谱显示在210、254和292 nm处有最大吸收峰。红外光谱显示在1675和1750 cm-1处有羰基，在1215 cm-1处有醚基。H - 1在短波或长波紫外光下均不发荧光，呈蓬松的白色晶体状，遇氢氧化铵或四乙烯五胺等碱性试剂会变黄。电子轰击和正化学电离的元素分析及精确质量测定表明其经验式为C23H24O8。其质谱（电子轰击、化学电离和快原子轰击[FAB]）显示分子离子峰为428，主要碎片离子峰在m/z + 386、368、355和295处。发现H - 1与由特异青霉（Penicillium wortmannii）和漆斑菌（Myrothecium roridum）产生的抗生素渥曼青霉素相同。这是关于镰刀菌属物种合成渥曼青霉素的首次报道。
