• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

产超广谱碳青霉烯酶高毒力.的分子流行病学

Molecular Epidemiology of Hypervirulent Carbapenemase-Producing .

机构信息

Department of Laboratory Medicine, Huashan Hospital, Fudan University, Shanghai, China.

Department of Intensive Care Unit, Huashan Hospital, Fudan University, Shanghai, China.

出版信息

Front Cell Infect Microbiol. 2021 Apr 7;11:661218. doi: 10.3389/fcimb.2021.661218. eCollection 2021.

DOI:10.3389/fcimb.2021.661218
PMID:33898334
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8058458/
Abstract

OBJECTIVE

To investigate the overall distributions of key virulence genes in , especially the hypervirulent -positive (Hv-(+)-KP).

METHODS

A total of 521 complete genomes of from GenBank were collected and analyzed. Multilocus sequence typing, molecular serotyping, antibiotic-resistance, virulence genes and plasmid replicon typing were investigated.

RESULTS

Positive rates of virulence genes highly varied, ranging from 2.9 () to 99.6% (). Totally 207 strains presented positive , , and and 190 showed positive , , , and , which were the two primary modes. A total of 94, 165 and 29 strains were denoted as hypervirulent (HvKP), (+)-KP and Hv-(+)-KP. ST11 accounted for 17 among the 29 Hv-(+)-KP strains; Genes , and were positive in 28, 26 and 18 Hv-(+)-KP strains respectively. Among the 29 Hv-(+)-KP strains exhibiting four super clusters from GenBank, IncHI1B plasmids carrying virulence genes and IncFII ones with were responsible for both 23 strains respectively.

CONCLUSIONS

Positive rates of virulence genes vary remarkably in . Genes , and were primary ones inducing Hv-(+)-KP. IncHI1B plasmids carrying virulence genes and IncFII ones with constitute the primary combination responsible for Hv-(+)-KP. The making of Hv-(+)-KP is mostly (+)-KP acquiring another plasmid harboring virulence genes.

摘要

目的

研究 中关键毒力基因的总体分布情况，尤其是高毒力 - 阳性（Hv-（+）-KP）。

方法

从 GenBank 中收集了 521 株完整的 基因组进行分析。对多位点序列分型、分子血清型、抗生素耐药性、毒力基因和质粒复制子类型进行了研究。

结果

毒力基因的阳性率差异很大，范围从 2.9（）到 99.6（）。共有 207 株呈阳性、、和，190 株呈阳性、、、和，这是两种主要模式。共有 94、165 和 29 株被定义为高毒力（HvKP）、（+）-KP 和 Hv-（+）-KP。在 29 株 Hv-（+）-KP 株中，ST11 占 17 株；28 株、26 株和 18 株 Hv-（+）-KP 株分别呈阳性。在来自 GenBank 的 29 株 Hv-（+）-KP 株中表现出四个超级聚类的菌株中，携带毒力基因的 IncHI1B 质粒和带有 的 IncFII 质粒分别负责 23 株。

结论

毒力基因在 中的阳性率差异很大。基因、和是诱导 Hv-（+）-KP 的主要因素。携带毒力基因的 IncHI1B 质粒和带有 的 IncFII 质粒构成了导致 Hv-（+）-KP 的主要组合。Hv-（+）-KP 的形成主要是（+）-KP 获得另一个携带毒力基因的质粒。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a793/8058458/5c4f862e9380/fcimb-11-661218-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a793/8058458/40d15b40f0b2/fcimb-11-661218-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a793/8058458/69edb895ce97/fcimb-11-661218-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a793/8058458/9887edd4e6a2/fcimb-11-661218-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a793/8058458/7821d3817373/fcimb-11-661218-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a793/8058458/2ea05871131a/fcimb-11-661218-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a793/8058458/36c4c5c4912a/fcimb-11-661218-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a793/8058458/95f2fc893604/fcimb-11-661218-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a793/8058458/5c4f862e9380/fcimb-11-661218-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a793/8058458/40d15b40f0b2/fcimb-11-661218-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a793/8058458/69edb895ce97/fcimb-11-661218-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a793/8058458/9887edd4e6a2/fcimb-11-661218-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a793/8058458/7821d3817373/fcimb-11-661218-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a793/8058458/2ea05871131a/fcimb-11-661218-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a793/8058458/36c4c5c4912a/fcimb-11-661218-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a793/8058458/95f2fc893604/fcimb-11-661218-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a793/8058458/5c4f862e9380/fcimb-11-661218-g008.jpg

相似文献

1
Molecular Epidemiology of Hypervirulent Carbapenemase-Producing .产超广谱碳青霉烯酶高毒力.的分子流行病学
Front Cell Infect Microbiol. 2021 Apr 7;11:661218. doi: 10.3389/fcimb.2021.661218. eCollection 2021.
2
Prevalence of Carbapenem-Resistant Hypervirulent and Hypervirulent Carbapenem-Resistant in China Determined Mouse Lethality Tests.中国通过小鼠致死性试验确定耐碳青霉烯类超级毒力和超级毒力耐碳青霉烯类的流行率。
Front Cell Infect Microbiol. 2022 Jun 1;12:882210. doi: 10.3389/fcimb.2022.882210. eCollection 2022.
3
Pandemic spread of bla among Klebsiella pneumoniae ST11 in China is associated with horizontal transfer mediated by IncFII-like plasmids.在中国，肺炎克雷伯菌 ST11 中 bla 的流行与 IncFII 样质粒介导的水平转移有关。
Int J Antimicrob Agents. 2019 Aug;54(2):117-124. doi: 10.1016/j.ijantimicag.2019.03.014. Epub 2019 Mar 15.
4
Emergence of Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae Coharboring a -Carrying Virulent Plasmid and a -Carrying Plasmid in an Egyptian Hospital.埃及医院中携带 - 碳青霉烯酶基因的高毒力耐碳青霉烯肺炎克雷伯菌的流行：携带毒力质粒和 - 碳青霉烯酶基因的质粒共同存在
mSphere. 2021 May 19;6(3):e00088-21. doi: 10.1128/mSphere.00088-21.
5
Cooccurrence of Antibiotic Resistance and Hypervirulence in High-Risk Carbapenem-Resistant K14.K64 and Wzi209 Klebsiella pneumoniae Strains Driven by Plasmids and Their Derivatives.高风险碳青霉烯类耐药 K14.K64 和 Wzi209 肺炎克雷伯菌菌株中由质粒及其衍生物驱动的抗生素耐药性和超毒力的共同发生。
Microbiol Spectr. 2022 Oct 26;10(5):e0254121. doi: 10.1128/spectrum.02541-21. Epub 2022 Aug 22.
6
Prevalence of Carbapenem-Resistant Co-Harboring blaKPC-Carrying Plasmid and pLVPK-Like Virulence Plasmid in Bloodstream Infections.血流感染中产碳青霉烯酶 blaKPC 携带质粒和 pLVPK 样毒力质粒的流行情况。
Front Cell Infect Microbiol. 2021 Mar 12;10:556654. doi: 10.3389/fcimb.2020.556654. eCollection 2020.
7
Genomic Analysis of KPC-2-Producing ST11 Isolates at the Respiratory Department of a Tertiary Care Hospital in Beijing, China.中国北京一家三级甲等医院呼吸科产KPC-2的ST11菌株的基因组分析
Front Microbiol. 2022 Jun 16;13:929826. doi: 10.3389/fmicb.2022.929826. eCollection 2022.
8
Antimicrobial Resistance Caused by KPC-2 Encoded by Promiscuous Plasmids of the Klebsiella pneumoniae ST307 Strain.由肺炎克雷伯菌 ST307 株的多功能质粒编码的 KPC-2 引起的抗生素耐药性。
Ann Lab Med. 2021 Jan;41(1):86-94. doi: 10.3343/alm.2021.41.1.86. Epub 2020 Aug 25.
9
KPC-2-encoding plasmids from Escherichia coli and Klebsiella pneumoniae in Taiwan.来自台湾大肠杆菌和肺炎克雷伯菌的携带KPC-2编码的质粒
J Antimicrob Chemother. 2014 Mar;69(3):628-31. doi: 10.1093/jac/dkt409. Epub 2013 Oct 11.
10
Emergence of an XDR and carbapenemase-producing hypervirulent Klebsiella pneumoniae strain in Taiwan.台湾出现产超广谱酶和碳青霉烯酶的高毒力肺炎克雷伯菌菌株。
J Antimicrob Chemother. 2018 Aug 1;73(8):2039-2046. doi: 10.1093/jac/dky164.

引用本文的文献

1
Urinary Multidrug-Resistant : Essential Oil Countermeasures in a One Health Case Report.泌尿道多重耐药：“同一健康”案例报告中的精油应对措施
Microorganisms. 2025 Aug 1;13(8):1807. doi: 10.3390/microorganisms13081807.
2
In vitro Evaluation of the Antibacterial Activity of the Combination of Avibactam and β-Lactams Against Highly Virulent Carbapenem-Resistant .阿维巴坦与β-内酰胺类药物联合对高毒力耐碳青霉烯类细菌抗菌活性的体外评价
Infect Drug Resist. 2025 Apr 26;18:2137-2152. doi: 10.2147/IDR.S515858. eCollection 2025.
3
Genomic characteristics, virulence potential, antimicrobial resistance profiles, and phylogenetic insights into Nocardia cyriacigeorgica.

本文引用的文献

1
The type I-E CRISPR-Cas system influences the acquisition of -IncF plasmid in .I 型 E CRISPR-Cas 系统影响. 中 -IncF 质粒的获得。
Emerg Microbes Infect. 2020 Dec;9(1):1011-1022. doi: 10.1080/22221751.2020.1763209.
2
Absence of the type I-E CRISPR-Cas system in Klebsiella pneumoniae clonal complex 258 is associated with dissemination of IncF epidemic resistance plasmids in this clonal complex.产Ⅰ-E 型 CRISPR-Cas 系统的缺失与肺炎克雷伯菌 258 克隆复合体中 IncF 流行耐药质粒的传播有关。
J Antimicrob Chemother. 2020 Apr 1;75(4):890-895. doi: 10.1093/jac/dkz538.
3
OrthoFinder: phylogenetic orthology inference for comparative genomics.
乔治西诺卡菌的基因组特征、毒力潜力、抗菌药物耐药谱及系统发育分析
Ann Clin Microbiol Antimicrob. 2025 Apr 5;24(1):22. doi: 10.1186/s12941-025-00791-x.
4
Molecular and genetic features of a bla and bla coharboring hypermucoviscous Klebsiella pneumoniae of serotype K2 and ST65.一株携带bla和bla的血清型K2和ST65的高黏液性肺炎克雷伯菌的分子和遗传特征
Ann Clin Microbiol Antimicrob. 2025 Mar 18;24(1):19. doi: 10.1186/s12941-025-00789-5.
5
Genomic characteristics and phylogenetic analyses of colonization and infection with carbapenem-resistant in multicenter intensive care units: a cohort study.多中心重症监护病房耐碳青霉烯类细菌定植与感染的基因组特征及系统发育分析：一项队列研究
Microbiol Spectr. 2025 Apr;13(4):e0158424. doi: 10.1128/spectrum.01584-24. Epub 2025 Feb 24.
6
Prevalence and molecular characteristics of colistin-resistant isolates among carbapenem-resistant Klebsiella pneumoniae in Central South China: a multicenter study.中国中南地区耐碳青霉烯类肺炎克雷伯菌中耐黏菌素分离株的流行情况及分子特征：一项多中心研究
Ann Clin Microbiol Antimicrob. 2025 Jan 4;24(1):1. doi: 10.1186/s12941-024-00769-1.
7
The Association between Resistance and Virulence of in High-Risk Clonal Lineages ST86 and ST101.高风险克隆谱系ST86和ST101中耐药性与毒力之间的关联
Microorganisms. 2024 Sep 30;12(10):1997. doi: 10.3390/microorganisms12101997.
8
Virulence Factors in : A Literature Review.《中的毒力因子：文献综述》 （注：原文中“Virulence Factors in :”表述不完整，这里根据常规推测补充了冒号后的内容，比如可能是某个具体领域，但由于原文缺失信息只能这样处理使译文尽量通顺。）
Indian J Microbiol. 2024 Jun;64(2):389-401. doi: 10.1007/s12088-024-01247-0. Epub 2024 Mar 27.
9
Analysis of virulence profiles in clinical isolates of from renal abscesses: clinical significance of hypervirulent isolates.从肾脓肿中分离的 临床分离株毒力谱分析：高毒力分离株的临床意义。
Front Cell Infect Microbiol. 2024 Mar 28;14:1367111. doi: 10.3389/fcimb.2024.1367111. eCollection 2024.
10
Development of a quadruple qRT-PCR assay for simultaneous identification of hypervirulent and carbapenem-resistant .建立一种四重 qRT-PCR 检测方法，用于同时鉴定高毒力和耐碳青霉烯的.
Microbiol Spectr. 2024 Jan 11;12(1):e0071923. doi: 10.1128/spectrum.00719-23. Epub 2023 Dec 7.
OrthoFinder：用于比较基因组学的系统发育直系同源推断。
Genome Biol. 2019 Nov 14;20(1):238. doi: 10.1186/s13059-019-1832-y.
4
Evolution of hypervirulence in carbapenem-resistant Klebsiella pneumoniae in China: a multicentre, molecular epidemiological analysis.中国碳青霉烯类耐药肺炎克雷伯菌高毒力进化：一项多中心、分子流行病学分析。
J Antimicrob Chemother. 2020 Feb 1;75(2):327-336. doi: 10.1093/jac/dkz446.
5
Hypervirulent Klebsiella pneumoniae - clinical and molecular perspectives.高毒力肺炎克雷伯菌：临床与分子视角
J Intern Med. 2020 Mar;287(3):283-300. doi: 10.1111/joim.13007. Epub 2019 Nov 21.
6
Plasmid evolution in carbapenemase-producing Enterobacteriaceae: a review.碳青霉烯酶产生肠杆菌科细菌中的质粒进化：综述。
Ann N Y Acad Sci. 2019 Dec;1457(1):61-91. doi: 10.1111/nyas.14223. Epub 2019 Aug 30.
7
Next-Generation Drug Discovery to Combat Antimicrobial Resistance.下一代药物发现以对抗抗微生物药物耐药性。
Trends Biochem Sci. 2019 Nov;44(11):961-972. doi: 10.1016/j.tibs.2019.05.005. Epub 2019 Jun 27.
8
Hypervirulent Klebsiella pneumoniae.高毒力肺炎克雷伯菌。
Clin Microbiol Rev. 2019 May 15;32(3). doi: 10.1128/CMR.00001-19. Print 2019 Jun 19.
9
Pandemic spread of bla among Klebsiella pneumoniae ST11 in China is associated with horizontal transfer mediated by IncFII-like plasmids.在中国，肺炎克雷伯菌 ST11 中 bla 的流行与 IncFII 样质粒介导的水平转移有关。
Int J Antimicrob Agents. 2019 Aug;54(2):117-124. doi: 10.1016/j.ijantimicag.2019.03.014. Epub 2019 Mar 15.
10
A Nationwide Screen of Carbapenem-Resistant Klebsiella pneumoniae Reveals an Isolate with Enhanced Virulence and Clinically Undetected Colistin Heteroresistance.全国范围内耐碳青霉烯类肺炎克雷伯菌的筛查揭示了一株具有增强毒力且临床无法检测到的多粘菌素异质性耐药的分离株。
Antimicrob Agents Chemother. 2019 Apr 25;63(5). doi: 10.1128/AAC.00107-19. Print 2019 May.