Savid Medical School, University of Chinese Academy of Sciences , Beijing, China.
Shenzhen Key Laboratory of Pathogen and Immunity, Shenzhen Clinical Research Center for infectious disease, Shenzhen Third People's Hospital, Second Hospital Affiliated to Southern University of Science and Technology , Shenzhen, Guangdong, China.
Microbiol Spectr. 2024 Jan 11;12(1):e0071923. doi: 10.1128/spectrum.00719-23. Epub 2023 Dec 7.
Globally, the increasing number of hypervirulent (hvKp) and carbapenem-resistant Kp (CR-Kp) infections poses a huge public health challenge with high morbidity and mortality. Worrisomely, due to the mobility of elements carrying virulence and drug-resistance genes, the increasing prevalence of CR-hvKp has also been found with an overwhelming mortality rate in recent years. However, the current detection methods for hvKp and CR-Kp have many disadvantages, such as long turnaround time, complex operation, low sensitivity, and specificity. Herein, a more sensitive, rapid, single-reaction, and multiplex quantitative real-time PCR was developed and validated to differentiate the circulating lineages of Kp with excellent performance in sensitivity and specificity, providing a useful tool for the differential diagnosis and the surveillance of the circulating Kp.
全球范围内，高毒力 (hvKp) 和耐碳青霉烯类肠杆菌科 (CR-Kp) 感染的数量不断增加，给公共卫生带来了巨大挑战，导致高发病率和高死亡率。令人担忧的是，由于携带毒力和耐药基因的元素的可移动性，近年来 CR-hvKp 的流行率也在不断增加，死亡率令人震惊。然而，目前 hvKp 和 CR-Kp 的检测方法存在许多缺点，例如检测时间长、操作复杂、灵敏度和特异性低。在此，开发并验证了一种更敏感、快速、单反应和多重实时定量 PCR 方法，用于区分肠杆菌科的流行株，该方法在灵敏度和特异性方面具有优异的性能，为鉴别诊断和监测流行的肠杆菌科提供了一种有用的工具。
