Nazer Mohammad Reza, Jahanbakhsh Sareh, Ebrahimi Katrin, Niazi Massumeh, Sepahvand Maryam, Khatami Mehrdad, Kharazi Sam
Lorestan University of Medical Sciences, Razi Herbal Medicine Research Center, Khorramabad, Iran
Lorestan University of Medical Sciences, Student Research Committee, Khorramabad, Iran
Turk J Pharm Sci. 2021 Apr 20;18(2):146-150. doi: 10.4274/tjps.galenos.2020.87259.
Cutaneous leishmania sis (CL) is considered as one of the most critical infections worldwide, in which the protozoa of the genus infects a person. Today, the common and selective drugs for the treatment of CL are antimonial compounds present some limitations to their usage. The objective of this study is to investigate the cytotoxic and antileishmanial effects of various extracts of L. on the model.
The primary phytochemical analysis of the extracts was performed to assess the presence of tannins, alkaloids, saponins, flavonoids, terpenoids, and glycosides. Furthermore, the in vitro cytotoxic and antileishmanial effects of extracts on promastigote were evaluated. Additionally, these effects on the J774-A1 macrophage cells by colorimetric cell viability 3-(4,5-dimethylthiazol- 2-yl)-2,5-diphenyl tetrazolium bromide assay were also assessed.
In this study, the findings of primary phytochemical screening of the extracts demonstrated the existence of flavonoids, tannins, terpenoids, glycosides, and alkaloids in this plant. Importantly, the findings indicated that the aqueous and methanolic extracts of exhibit a high potency to inhibit the growth of promastigotes with inhibitory concentration 50 values of aqueous and methanolic extracts being 28.5 and 44.6 μg/mL, respectively. Based on the obtained results, extracts did not display a considerable cytotoxicity on the J774-A1 macrophage cells.
The obtained findings exhibited remarkable antileishmanial effects of extracts on , thereby indicating the ability of as a herbal product to be developed as a new antileishmanial drug. Nevertheless, supplementary investigations will be obligatory to achieve these findings, especially in human subjects.
皮肤利什曼病（CL）被认为是全球最严重的感染之一，利什曼原虫属的原生动物感染人体。如今，治疗CL的常用和选择性药物是锑化合物，但它们的使用存在一些局限性。本研究的目的是研究L.的各种提取物对该模型的细胞毒性和抗利什曼原虫作用。
对提取物进行初步植物化学分析，以评估单宁、生物碱、皂苷、黄酮类化合物、萜类化合物和糖苷的存在。此外，评估提取物对前鞭毛体的体外细胞毒性和抗利什曼原虫作用。另外，还通过比色细胞活力3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑试验评估这些提取物对J774-A1巨噬细胞的影响。
在本研究中，提取物初步植物化学筛选的结果表明该植物中存在黄酮类化合物、单宁、萜类化合物、糖苷和生物碱。重要的是，结果表明L.的水提取物和甲醇提取物具有高效抑制前鞭毛体生长的能力，水提取物和甲醇提取物的半数抑制浓度值分别为28.5和44.6μg/mL。根据所得结果，提取物对J774-A1巨噬细胞没有表现出明显的细胞毒性。
所得结果显示L.提取物对具有显著的抗利什曼原虫作用，从而表明L.作为一种草药产品有能力被开发为一种新型抗利什曼原虫药物。然而，要实现这些结果还需要进行补充研究，尤其是在人体受试者中。
