Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States; Department of Environmental and Global Health, College of Public Health and Health Professions, University of Florida, Gainesville, FL, United States.
Emerging Pathogens Institute, University of Florida, Gainesville, FL, United States; Department of Medicine, College of Medicine, University of Florida, Gainesville, FL, United States.
Int J Infect Dis. 2021 Jul;108:212-216. doi: 10.1016/j.ijid.2021.04.063. Epub 2021 Apr 24.
To determine if viable virus could be isolated from the air within a car driven by a patient infected with SARS-CoV-2, and to assess the size range of the infectious particles.
We used a Sioutas personal cascade impactor sampler (PCIS) to screen for SARS-CoV-2 in a car driven by a COVID-19 patient. The patient, who had only mild illness without fever or cough and was not wearing a mask, drove the car for 15 min with the air conditioning turned on and windows closed. The PCIS was clipped to the sun-visor above the front passenger seat and was retrieved from the car two hours after completion of the drive.
SARS-CoV-2 was detectable at all PCIS stages by PCR and was cultured from the section of the sampler collecting particles in the 0.25-0.50 μm size range.
Our data highlight the potential risk of SARS-CoV-2 transmission by minimally symptomatic persons in the closed space inside of a car and suggest that a substantial component of that risk is via aerosolized virus.
确定在 SARS-CoV-2 感染患者驾驶的车内空气中是否可以分离到有活力的病毒，并评估感染性颗粒的大小范围。
我们使用 Sioutas 个人级联冲击采样器（PCIS）对 COVID-19 患者驾驶的汽车内的 SARS-CoV-2 进行筛查。该患者仅患有轻度疾病，没有发热或咳嗽，也未佩戴口罩，在空调开启且车窗关闭的情况下驾驶汽车 15 分钟。PCIS 夹在前排乘客座椅上方的遮阳板上，在驾驶结束两小时后从车内取回。
通过 PCR 在所有 PCIS 级联中均检测到 SARS-CoV-2，并且从收集 0.25-0.50μm 大小范围内颗粒的采样器部分培养出了 SARS-CoV-2。
我们的数据突出了症状轻微的人在汽车内部封闭空间中传播 SARS-CoV-2 的潜在风险，并表明该风险的很大一部分是通过气溶胶化的病毒。
