Alam Md Sarowar, Tester Mark, Fiene Gabriele, Mousa Magdi Ali Ahmed
Department of Arid Land Agriculture, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Plant Breeding Division, Bangladesh Agricultural Research Institute (BARI), Gazipur 1701, Bangladesh.
Plants (Basel). 2021 Apr 7;10(4):712. doi: 10.3390/plants10040712.
Salinity is one of the most significant environmental stresses for sustainable crop production in major arable lands of the globe. Thus, we conducted experiments with 27 tomato genotypes to screen for salinity tolerance at seedling stage, which were treated with non-salinized (S1) control (18.2 mM NaCl) and salinized (S2) (200 mM NaCl) irrigation water. In all genotypes, the elevated salinity treatment contributed to a major depression in morphological and physiological characteristics; however, a smaller decrease was found in certain tolerant genotypes. Principal component analyses (PCA) and clustering with percentage reduction in growth parameters and different salt tolerance indices classified the tomato accessions into five key clusters. In particular, the tolerant genotypes were assembled into one cluster. The growth and tolerance indices PCA also showed the order of salt-tolerance of the studied genotypes, where Saniora was the most tolerant genotype and P.Guyu was the most susceptible genotype. To investigate the possible biochemical basis for salt stress tolerance, we further characterized six tomato genotypes with varying levels of salinity tolerance. A higher increase in proline content, and antioxidants activities were observed for the salt-tolerant genotypes in comparison to the susceptible genotypes. Salt-tolerant genotypes identified in this work herald a promising source in the tomato improvement program or for grafting as scions with improved salinity tolerance in tomato.
盐分是全球主要耕地可持续作物生产面临的最重大环境胁迫之一。因此，我们对27种番茄基因型进行了实验，以筛选其在幼苗期的耐盐性，这些基因型分别用非盐渍化（S1）对照（18.2 mM NaCl）和盐渍化（S2）（200 mM NaCl）灌溉水进行处理。在所有基因型中，盐分升高处理导致形态和生理特征大幅下降；然而，在某些耐盐基因型中下降幅度较小。主成分分析（PCA）以及根据生长参数和不同耐盐指数的降幅进行聚类，将番茄品种分为五个关键类别。特别是，耐盐基因型被归为一类。生长和耐盐指数的主成分分析还显示了所研究基因型的耐盐顺序，其中Saniora是最耐盐的基因型，而P.Guyu是最敏感的基因型。为了探究耐盐胁迫可能的生化基础，我们进一步对六种耐盐性不同的番茄基因型进行了表征。与敏感基因型相比，耐盐基因型的脯氨酸含量和抗氧化剂活性有更高的增加。本研究中鉴定出的耐盐基因型为番茄改良计划或作为具有更高耐盐性的接穗进行嫁接提供了一个有前景的资源。
