Habibi Nasratullah, Aryan Shafiqullah, Amin Mohammad Wasif, Sanada Atsushi, Terada Naoki, Koshio Kaihei
Graduate School of Agriculture, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo 156-8502, Japan.
Faculty of Agriculture, Balkh University, Balkh 1701, Afghanistan.
Plants (Basel). 2023 May 31;12(11):2187. doi: 10.3390/plants12112187.
Pre-sowing seed priming is one of the methods used to improve the performance of tomato plants under salt stress, but its effect photosynthesis, yield, and quality have not yet been well investigated. This experiment aimed to alleviate the impact of sodium chloride stress on the photosynthesis parameters of tomato cv. Micro-Tom (a dwarf L.) plants exposed to salt stress conditions. Each treatment combination consisted of five different sodium chloride concentrations (0 mM, 50 mM, 100 mM, 150 mM, and 200 mM) and four priming treatments (0 MPa, -0.4 MPa, -0.8 MPa, and -1.2 MPa), with five replications. Microtome seeds were subjected to polyethylene glycol (PEG6000) treatments for 48 hours for priming, followed by germination on a moist filter paper, and then transferred to the germination bed after 24 h. Subsequently, the seedlings were transplanted into the Rockwool, and the salinity treatments were administered after a month. In our study salinity significantly affected tomato plants' physiological and antioxidant attributes. Primed seeds produced plants that exhibited relatively better photosynthetic activity than those grown from unprimed seeds. Our findings indicated that priming doses of -0.8 MPa and -1.2 MPa were the most effective at stimulating tomato plant photosynthesis, and biochemical contents under salinity-related conditions. Moreover, primed plants demonstrated relatively superior fruit quality features such as fruit color, fruit Brix, sugars (glucose, fructose, and sucrose), organic acids, and vitamin C contents under salt stress, compared to non-primed plants. Furthermore, priming treatments significantly decreased the malondialdehyde, proline, and hydrogen peroxide content in plant leaves. Our results suggest that seed priming may be a long-term method for improving crop productivity and quality in challenging environments by enhancing the growth, physiological responses, and fruit quality attributes of Micro-Tom tomato plants under salt stress conditions.
播种前种子引发是用于提高番茄植株在盐胁迫下性能的方法之一，但其对光合作用、产量和品质的影响尚未得到充分研究。本试验旨在减轻氯化钠胁迫对盐胁迫条件下番茄品种Micro-Tom（矮生番茄）植株光合作用参数的影响。每个处理组合包括五种不同的氯化钠浓度（0 mM、50 mM、100 mM、150 mM和200 mM）和四种引发处理（0 MPa、-0.4 MPa、-0.8 MPa和-1.2 MPa），每个处理设置五个重复。将Micro-Tom种子用聚乙二醇（PEG6000）处理48小时进行引发，随后在湿润滤纸上萌发，24小时后转移至发芽床。之后，将幼苗移栽到岩棉中，一个月后进行盐度处理。在我们的研究中，盐度显著影响番茄植株的生理和抗氧化特性。引发处理的种子培育出的植株比未引发处理种子培育出的植株表现出相对更好的光合活性。我们的研究结果表明，-0.8 MPa和-1.2 MPa的引发剂量在刺激盐胁迫相关条件下番茄植株光合作用和生化含量方面最为有效。此外，与未引发处理的植株相比，引发处理的植株在盐胁迫下果实品质特征相对更优，例如果实颜色、果实糖度、糖类（葡萄糖、果糖和蔗糖）、有机酸和维生素C含量。此外，引发处理显著降低了植物叶片中丙二醛、脯氨酸和过氧化氢的含量。我们的结果表明，种子引发可能是一种通过增强盐胁迫条件下Micro-Tom番茄植株的生长、生理反应和果实品质属性来提高挑战性环境中作物生产力和品质的长效方法。
