Salayová Aneta, Bedlovičová Zdenka, Daneu Nina, Baláž Matej, Lukáčová Bujňáková Zdenka, Balážová Ľudmila, Tkáčiková Ľudmila
Department of Chemistry, Biochemistry and Biophysics, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovakia.
Advanced Materials Department, Jožef Stefan Institute, Jamova cesta 39, 1000 Ljubljana, Slovenia.
Nanomaterials (Basel). 2021 Apr 14;11(4):1005. doi: 10.3390/nano11041005.
A green synthetic route for the production of silver nanoparticles (AgNPs) using five different aqueous plant extracts, namely, , , , and , was investigated in this study. The present work demonstrates the influence of plant extract composition (antioxidant and total phenolic content) on the size and morphology of the produced AgNPs. The biosynthetic procedure was rapid and simple and was easily monitored via colour changes and ultraviolet and visible (UV-Vis) spectroscopy. Subsequently, measurement of zeta potential (ZP), photon cross-correlation spectroscopy (PCCS), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM) and selected area electron diffraction (SAED) analysis were employed to characterise the as-synthesised nanoparticles. The XRD investigation confirmed the presence of Ag in the nanoparticles, and interactions between the bioactive compounds of the plants and the produced AgNPs were evident in the FTIR spectra. TEM indicated that the nanoparticles exhibited a bimodal size distribution, with the smaller particles being spherical and the larger having a truncated octahedron shape. In addition, the antimicrobial activity of the AgNPs was tested against five bacterial strains. All synthesised nanoparticles exhibited enhanced antimicrobial activity at a precursor concentration of 5 mM compared to the control substance, gentamicin sulphate, with the best results observed for AgNPs prepared with and extracts.
本研究探索了一条利用五种不同的植物水提取物（即 、 、 、 和 ）来制备银纳米颗粒（AgNPs）的绿色合成路线。目前的工作证明了植物提取物成分（抗氧化剂和总酚含量）对所制备的AgNPs的尺寸和形态的影响。生物合成过程快速且简单，通过颜色变化以及紫外可见（UV-Vis）光谱能够轻松监测。随后，采用测量zeta电位（ZP）、光子互相关光谱（PCCS）、X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、透射电子显微镜（TEM）和选区电子衍射（SAED）分析对合成后的纳米颗粒进行表征。XRD研究证实了纳米颗粒中存在银，并且植物生物活性化合物与所制备的AgNPs之间的相互作用在FTIR光谱中很明显。TEM表明纳米颗粒呈现双峰尺寸分布，较小的颗粒呈球形，较大的颗粒呈截角八面体形状。此外，测试了AgNPs对五种细菌菌株的抗菌活性。与对照物质硫酸庆大霉素相比，所有合成的纳米颗粒在前体浓度为5 mM时均表现出增强的抗菌活性，其中用 和 提取物制备的AgNPs效果最佳。
