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INTRODUCTION

This study explores the biogenic synthesis of silver nanoparticles (AgNPs) using keratinase from Pseudomonas aeruginosa-C1M as a reducing and stabilizing agent. The synthesis of AgNPs was characterized by a color change from transparent to dark brown and a UV-Vis absorption peak at 450 nm, confirming nanoparticle formation. The study further investigates the structural, morphological, and functional properties of these AgNPs, particularly their antibacterial activity and their potential role in azo dye decontamination.

METHODS AND RESULTS

The FTIR confirmed that AgNPs nanoparticles formation with keratinase. X-ray diffraction analysis showed that the prepared AgNPs were crystalline in nature and had face-centered cubic lattice planes. When observed under the transmission electron microscope and scanning electron microscope the nanoparticles were monodispersed spheres of different sizes. The diameter of the AgNPs was ~ 119 nm according to dynamic light scattering. High dispersion, long-term stability and excellent colloidal properties were supported by a high negative zeta potential value. The silver nanoparticles were found to have an antibacterial activity with zone of inhibition 25 mm and 33 mm against pathogenic strains of Staphylococcus aureus and Escherichia coli respectively. The synthesized zero-valent silver nanoparticles assisted in the decontamination of azo dyes (methyl red, methyl orange, safranin O and methyl violet) through the incorporation of sodium borohydride and light-catalyzed processes.

CONCLUSION

This study demonstrates, for the first time, that keratinase from Pseudomonas aeruginosa-C1M can be used for AgNPs synthesis. The biogenic AgNPs exhibited potent antibacterial activity and played a crucial role in detoxifying hazardous azo dyes. These findings highlight the dual-functional potential of AgNPs for applications in antimicrobial treatments and environmental remediation. Future studies should explore their mechanism of action, scalability, and industrial applications.

INTRODUCTION

METHODS AND RESULTS

CONCLUSION

引言

本研究探索了以铜绿假单胞菌 - C1M产生的角蛋白酶作为还原剂和稳定剂进行银纳米颗粒（AgNPs）的生物合成。AgNPs的合成通过颜色从透明变为深棕色以及在450 nm处的紫外 - 可见吸收峰来表征，证实了纳米颗粒的形成。该研究进一步调查了这些AgNPs的结构、形态和功能特性，特别是它们的抗菌活性以及在偶氮染料去污中的潜在作用。

方法与结果

傅里叶变换红外光谱（FTIR）证实了角蛋白酶参与了AgNPs的形成。X射线衍射分析表明，制备的AgNPs本质上是晶体，具有面心立方晶格平面。在透射电子显微镜和扫描电子显微镜下观察时，纳米颗粒是不同大小的单分散球体。根据动态光散射，AgNPs的直径约为119 nm。高负的zeta电位值表明其具有高分散性、长期稳定性和优异的胶体性质。发现银纳米颗粒对金黄色葡萄球菌和大肠杆菌的致病菌株具有抗菌活性，抑菌圈分别为25 mm和33 mm。合成的零价银纳米颗粒通过加入硼氢化钠和光催化过程协助偶氮染料（甲基红、甲基橙、番红O和甲基紫）的去污。