Tufail Muhammad Aammar, Touceda-González María, Pertot Ilaria, Ehlers Ralf-Udo
Department of Civil, Environmental and Mechanical Engineering, University of Trento, Via Mesiano 77, 38123 Trento, Italy.
e-nema Gesellschaft für Biotechnologie und Biologischen Pflanzenschutz mbH, Klausdorfer Str. 28-36, 24223 Schwentinental, Germany.
Microorganisms. 2021 Apr 17;9(4):870. doi: 10.3390/microorganisms9040870.
Plant growth promoting endophytic bacteria, which can fix nitrogen, plays a vital role in plant growth promotion. Previous authors have evaluated the effect of Pal5 inoculation on plants subjected to different sources of abiotic stress on an individual basis. The present study aimed to appraise the effect of inoculation on the amelioration of the individual and combined effects of drought and nitrogen stress in maize plants ( L.). A pot experiment was conducted whereby treatments consisted of maize plants cultivated under drought stress, in soil with a low nitrogen concentration and these two stress sources combined, with and without seed inoculation. The inoculated plants showed increased plant biomass, chlorophyll content, plant nitrogen uptake, and water use efficiency. A general increase in copy numbers of , based on 16S rRNA gene quantification, was detected under combined moderate stress, in addition to an increase in the abundance of genes involved in N fixation (). Endophytic colonization of bacteria was negatively affected by severe stress treatments. Overall, Pal5 can be considered as an effective tool to increase maize crop production under drought conditions with low application of nitrogen fertilizer.
促进植物生长的内生细菌能够固氮，在促进植物生长方面发挥着至关重要的作用。此前的作者已分别评估了Pal5接种对遭受不同非生物胁迫源的植物的影响。本研究旨在评估接种对缓解玉米植株干旱和氮胁迫的单一及综合影响的作用。进行了一项盆栽试验，处理包括在干旱胁迫、低氮浓度土壤以及这两种胁迫源组合条件下种植的玉米植株，且有接种种子和未接种种子之分。接种的植株表现出植物生物量增加、叶绿素含量增加、植物氮吸收增加以及水分利用效率提高。基于16S rRNA基因定量分析，在中度复合胁迫下检测到细菌拷贝数普遍增加，此外参与固氮的基因丰度也有所增加。重度胁迫处理对细菌的内生定殖产生了负面影响。总体而言，Pal5可被视为在低氮肥施用量的干旱条件下提高玉米作物产量的有效工具。
